Literature DB >> 6237594

A simplified procedure for synthesizing large quantities of highly purified uridine [beta-32P]diphospho-N-acetylglucosamine.

L Lang, S Kornfeld.   

Abstract

A simplified procedure for the synthesis of [beta-32P]UDP-N-acetylglucosamine is described. This novel method utilizes commercially available enzymes, chemical acetylation, and preparative thin-layer chromatography and can be used to synthesize millicurie quantities of the sugar nucleotide at an extremely high specific activity and purity.

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Year:  1984        PMID： 6237594     DOI： 10.1016/0003-2697(84)90163-5

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  14 in total

Review 1.  Enzymatic synthesis of nucleotide sugars.

Authors:  T Bülter; L Elling
Journal:  Glycoconj J       Date:  1999-02       Impact factor: 2.916

2.  Four monoclonal antibodies inhibit the recognition of arylsulphatase A by the lysosomal enzyme phosphotransferase.

Authors:  H J Sommerlade; A Hille-Rehfeld; K von Figura; V Gieselmann
Journal:  Biochem J       Date:  1994-01-01       Impact factor: 3.857

3.  Altered molecular size of N-acetylglucosamine 1-phosphotransferase in I-cell disease and pseudo-Hurler polydystrophy.

Authors:  Y Ben-Yoseph; M Potier; D A Mitchell; B A Pack; S B Melançon; H L Nadler
Journal:  Biochem J       Date:  1987-12-15       Impact factor: 3.857

4.  Characterization of the mannose 6-phosphate-dependent pathway of lysosomal enzyme routing in an invertebrate.

Authors:  V Alvarez; A J Parodi; R Couso
Journal:  Biochem J       Date:  1995-09-01       Impact factor: 3.857

5.  Cytosolic ARFs are required for vesicle formation but not for cell-free intra-Golgi transport: evidence for coated vesicle-independent transport.

Authors:  T C Taylor; M Kanstein; P Weidman; P Melançon
Journal:  Mol Biol Cell       Date:  1994-02       Impact factor: 4.138

6.  Lysosomal enzyme phosphorylation in human fibroblasts. Kinetic parameters offer a biochemical rationale for two distinct defects in the uridine diphospho-N-acetylglucosamine:lysosomal enzyme precursor N-acetylglucosamine-1-phosphotransferase.

Authors:  L Lang; T Takahashi; J Tang; S Kornfeld
Journal:  J Clin Invest       Date:  1985-12       Impact factor: 14.808

7.  Formation of UDP-2-acetamido-2-deoxy-L-galactose and UDP-2-acetamido-2-deoxy-L-galacturonic acid by Pseudomonas aeruginosa.

Authors:  S Singh; U Singh; S E Hogan; D S Feingold
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490

8.  Acyl-acyl carrier protein specificity of UDP-GlcNAc acyltransferases from gram-negative bacteria: relationship to lipid A structure.

Authors:  J M Williamson; M S Anderson; C R Raetz
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

9.  Yeast and mammals utilize similar cytosolic components to drive protein transport through the Golgi complex.

Authors:  W G Dunphy; S R Pfeffer; D O Clary; B W Wattenberg; B S Glick; J E Rothman
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205

10.  Mucolipidoses II and III variants with normal N-acetylglucosamine 1-phosphotransferase activity toward alpha-methylmannoside are due to nonallelic mutations.

Authors:  Y Ben-Yoseph; D A Mitchell; R M Yager; J T Wei; T H Chen; L Y Shih
Journal:  Am J Hum Genet       Date:  1992-01       Impact factor: 11.025
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