Literature DB >> 6235810

Direct radioimmunoassay of atrial natriuretic factor.

J Gutkowska, G Thibault, P Januszewicz, M Cantin, J Genest.   

Abstract

A direct radioimmunoassay of atrial natriuretic factor (ANF) has been developed. The method uses a synthetic 26 amino-acid fragment (8-33 ANF) of the native peptide. Antibodies have been prepared in rabbits immunized with the peptide coupled to thyroglobulin. The radiolabelled tracer prepared by iodination according to the Chloramine-T method has been purified by HPLC followed by affinity chromatography on Sepharose-4B anti-ANF. Dextran-coated charcoal has been used for separation of free from antibody bound radioactivity. Higher ANF content has been found in the right rat atrium than in the left. These results have been confirmed by bioassay.

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Year:  1984        PMID: 6235810     DOI: 10.1016/s0006-291x(84)80074-1

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN: 0006-291X            Impact factor:   3.575


  23 in total

1.  Antibodies against amino acids 1-15 of tumor necrosis factor block its binding to cell-surface receptor.

Authors:  S H Socher; M W Riemen; D Martinez; A Friedman; J Tai; J C Quintero; V Garsky; A Oliff
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

2.  Atrial-specific granules in the hearts of normal and water-deprived rats.

Authors:  H Toshimori; K Toshimori; S Matsukura; C Oura; H Matsuo
Journal:  Cell Tissue Res       Date:  1988-09       Impact factor: 5.249

3.  Hypoxia regulates the natriuretic peptide system.

Authors:  Olli Arjamaa; Mikko Nikinmaa
Journal:  Int J Physiol Pathophysiol Pharmacol       Date:  2011-09-07

4.  Extra-atrial expression of the gene for atrial natriuretic factor.

Authors:  D G Gardner; C F Deschepper; W F Ganong; S Hane; J Fiddes; J D Baxter; J Lewicki
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

5.  Contribution of blood and systemic circulation to the processing of pro-(atrial natriuretic factor).

Authors:  K K Murthy; G Thibault; M Cantin
Journal:  Biochem J       Date:  1988-03-15       Impact factor: 3.857

Review 6.  The biochemistry and molecular biology of atrial natriuretic factor.

Authors:  T G Flynn; P L Davies
Journal:  Biochem J       Date:  1985-12-01       Impact factor: 3.857

7.  The atrial natriuretic factor in mild essential hypertension.

Authors:  J Genest; P Larochelle; J R Cusson; J Gutkowska; M Cantin; R Garcia; G Thibault; O Kuchel; A De Léan; P Hamet
Journal:  Trans Am Clin Climatol Assoc       Date:  1987

8.  Time course of blood volume change with carbon monoxide inhalation and its contribution to the overall cardiovascular response.

Authors:  S B Davidson; D G Penney
Journal:  Arch Toxicol       Date:  1988       Impact factor: 5.153

9.  Mechanisms of transthyretin amyloidogenesis. Antigenic mapping of transthyretin purified from plasma and amyloid fibrils and within in situ tissue localizations.

Authors:  A Gustavsson; U Engström; P Westermark
Journal:  Am J Pathol       Date:  1994-06       Impact factor: 4.307

10.  Central nitrergic system regulation of neuroendocrine secretion, fluid intake and blood pressure induced by angiotensin-II.

Authors:  Wagner L Reis; Wilson A Saad; Luiz A Camargo; Lucila Lk Elias; José Antunes-Rodrigues
Journal:  Behav Brain Funct       Date:  2010-10-25       Impact factor: 3.759

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