Identification of the alpha and beta subunits of the chloroplast coupling factor one in Chlamydomonas reinhardi.

The alpha and beta subunits of the Chlamydomonas reinhardi coupling factor one have been identified by an immunochemical method and by the reaction of dicyclohexylcarbodiimide with the beta subunit. Antibodies raised against the C. reinhardi subunit with the highest apparent molecular weight react with the alpha subunit of spinach coupling factor one and antibodies raised against the beta subunit of the spinach chloroplast enzyme cross-react with the C. reinhardi subunit of lower apparent molecular weight. Dicyclohexylcarbodiimide also reacts with this subunit. We conclude therefore that the two subunits of highest apparent molecular weight can be named alpha and beta in order of decreasing apparent molecular weight, in contrast to the nomenclature suggested by Piccioni, R. G., Bennoun, P. and Chua, N.-H. [(1981) Eur. J. Biochem. 117, 93-102].