| Literature DB >> 6226538 |
Abstract
ATP-dependent calcium uptake was studied in isolated guinea pig parotid gland microsomes. The apparent Km for free Ca2+ was 0.41, microM, the apparent Km for ATP X Mg2- 0.23 mM. The pH optimum was 6.8-7.0. Subfractionation of the microsomes revealed that the highest specific uptake activity resided in a rather dense fraction of the endoplasmic reticulum. The calcium uptake/ATPase stoichiometry was determined in the absence of exogenous magnesium in the submicrosomal fractions. It ranged from 1-2. It is concluded that in vivo the stoichiometry is the same as in sarcoplasmic reticulum, namely 2.Entities:
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Year: 1983 PMID： 6226538 DOI： 10.1016/0014-5793(83)80796-0
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124