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Literature DB >> 6222732

Some properties of the Ca2+-stimulated ATPase of a rat liver microsomal fraction.

Abstract

1. The heavy microsomal fraction from rat liver apparently has very little Ca2+-stimulated ATPase activity, although it has an active, ATP-driven Ca2+ accumulation system. 2. The addition of ionophore A23187 to the ATPase assay, to allow continuous Ca2+ recycling during the assay time, reveals the presence of a substantial Ca2+-stimulated ATPase with Vmax. 160 nmol of Pi/10 min per mg of protein and Km for Ca2+ 0.19 microM. 3. The Ca2+-stimulated ATPase, but not the basal Mg2+-stimulated ATPase, is potently inhibited by orthovanadate. Both the Ca2+-stimulated ATPase and the vanadate inhibition are enhanced by the presence of Mg2+. 4. Ca2+-stimulated ATPase activity is not responsive to calmodulin or the calmodulin antagonist trifluoperazine.

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Year: 1983 PMID： 6222732 PMCID： PMC1154238 DOI： 10.1042/bj2100405

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

18 in total

1. Isolation of a potent (Na-K)ATPase inhibitor from striated muscle.

Authors: L Josephson; L C Cantley
Journal: Biochemistry Date: 1977-10-18 Impact factor: 3.162

2. Effects of potassium on vanadate inhibition of sarcoplasmic reticulum Ca2+-ATPase from dog cardiac and rabbit skeletal muscle.

Authors: T Wang; L I Tsai; R J Solaro; A O Grassi de Gende; A Schwartz
Journal: Biochem Biophys Res Commun Date: 1979-11-14 Impact factor: 3.575

3. Partial purification of the Ca2+-Mg2+ ATPase activator from human erythrocytes: its similarity to the activator of 3':5' - cyclic nucleotide phosphodiesterase.

Authors: H W Jarrett; J T Penniston
Journal: Biochem Biophys Res Commun Date: 1977-08-22 Impact factor: 3.575

4. Phosphodiesterase protein activator mimics red blood cell cytoplasmic activator of (Ca2+-Mg2+)ATPase.

Authors: R M Gopinath; F F Vincenzi
Journal: Biochem Biophys Res Commun Date: 1977-08-22 Impact factor: 3.575

5. Calmodulin in natural and reconstituted calcium transporting systems.

Authors: E Carafoli; V Niggli; K Malmström; P Caroni
Journal: Ann N Y Acad Sci Date: 1980 Impact factor: 5.691

6. Inhibition of red cell Ca2+-ATPase by vanadate.

Authors: G H Bond; P M Hudgins
Journal: Biochim Biophys Acta Date: 1980-08-14

7. Properties of energy-dependent calcium transport by rat liver microsomal fraction as revealed by initial-rate measurements.

Authors: F L Bygrave
Journal: Biochem J Date: 1978-01-15 Impact factor: 3.857

8. Energy-dependent calcium sequestration activity in rat liver microsomes.

Authors: L Moore; T Chen; H R Knapp; E J Landon
Journal: J Biol Chem Date: 1975-06-25 Impact factor: 5.157

9. Characterization of the hormone-sensitive Ca2+ uptake activity of the hepatic endoplasmic reticulum.

Authors: A M Andia-Waltenbaugh; A Lam; L Hummel; N Friedmann
Journal: Biochim Biophys Acta Date: 1980-06-19

10. The Ca2+-pumping ATPase of heart sarcolemma. Characterization, calmodulin dependence, and partial purification.

Authors: P Caroni; E Carafoli
Journal: J Biol Chem Date: 1981-04-10 Impact factor: 5.157

12 in total

1. Does a calmodulin-dependent Ca2+-regulated Mg2+-dependent ATPase contribute to hepatic microsomal calcium uptake?

Authors: S Schütze; H D Söling
Journal: Biochem J Date: 1987-05-01 Impact factor: 3.857

2. The mechanism of action of GTP on Ca2+ efflux from rat liver microsomal vesicles. Measurement of vesicle fusion by fluorescence energy transfer.

Authors: J G Comerford; A P Dawson
Journal: Biochem J Date: 1988-01-01 Impact factor: 3.857

3. Thapsigargin, a tumor promoter, discharges intracellular Ca2+ stores by specific inhibition of the endoplasmic reticulum Ca2(+)-ATPase.

Authors: O Thastrup; P J Cullen; B K Drøbak; M R Hanley; A P Dawson
Journal: Proc Natl Acad Sci U S A Date: 1990-04 Impact factor: 11.205

4. Characterization of Ca Transport in Purified Endoplasmic Reticulum Membrane Vesicles from Lepidium sativum L. Roots.

Authors: T J Buckhout
Journal: Plant Physiol Date: 1984-12 Impact factor: 8.340

5. Purification of the microsomal Ca2(+)-ATPase from rat liver.

Authors: Y J Jong; A Sheldon; G H Zhang; N Kraus-Friedmann
Journal: J Membr Biol Date: 1990-10 Impact factor: 1.843

6. A procedure for the rapid isolation from rat liver of plasma membrane vesicles exhibiting Ca2+-transport and Ca2+-ATPase activities.

Authors: R J Epping; F L Bygrave
Journal: Biochem J Date: 1984-11-01 Impact factor: 3.857

7. A steady-state mechanism can account for the properties of inositol 2,4,5-trisphosphate-stimulated Ca2+ release from permeabilized L1210 cells.

Authors: J W Loomis-Husselbee; A P Dawson
Journal: Biochem J Date: 1993-02-01 Impact factor: 3.857