Literature DB >> 6222657

Renal localization of heparan sulfate proteoglycan by immunohistochemistry.

D J Klein, T R Oegema, R Eisenstein, L Furcht, A F Michael, D M Brown.   

Abstract

Glomerular localization of heparan sulfate proteoglycan (HS-proteoglycan) has been studied immunohistochemically with a highly purified antiserum to bovine aorta HS-proteoglycan core protein. The specificity of the antiserum was enhanced by consecutive fibronectin and chondroitin sulfate-dermatan sulfate proteoglycan (CS-DS proteoglycan) affinity chromatography. The affinity-purified HS-proteoglycan antibody lacked cross-reactivity by enzyme-linked immunosorbent assays (ELISA) with CS-DS proteoglycan, fibronectin, laminin, and Type IV collagen. Reactivity of the antiserum with HS-proteoglycan antigen by ELISA was inhibited by HS core protein derived from CsCl density gradient centrifugation after heparinase treatment of the HS-proteoglycan. Immunofluorescent reactivity of the HS-proteoglycan antiserum was observed with bovine glomerular basement membrane, renal interstitium, Bowman's capsule, renal arterioles, and bovine aorta. No staining was seen with rat, mouse, or human glomeruli.

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Year:  1983        PMID： 6222657      PMCID： PMC1916277     

Source DB:  PubMed          Journal:  Am J Pathol        ISSN： 0002-9440            Impact factor:   4.307


  35 in total

1.  Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Authors:  E Engvall; P Perlmann
Journal:  J Immunol       Date:  1972-07       Impact factor: 5.422

2.  A simplified method for cyanogen bromide activation of agarose for affinity chromatography.

Authors:  S C March; I Parikh; P Cuatrecasas
Journal:  Anal Biochem       Date:  1974-07       Impact factor: 3.365

3.  Characterization of bovine aorta proteoglycan extracted with guanidine hydrochloride in the presence of protease inhibitors.

Authors:  T R Oegema; V C Hascall; R Eisenstein
Journal:  J Biol Chem       Date:  1979-02-25       Impact factor: 5.157

4.  Molecular basis of proteinuria of glomerular origin.

Authors:  B M Brenner; T H Hostetter; H D Humes
Journal:  N Engl J Med       Date:  1978-04-13       Impact factor: 91.245

5.  The ground substance of the arterial wall. Part 1. Extractability of glycosaminoglycans and the isolation of a proteoglycan from bovine aorta.

Authors:  R Eisenstein; S E Larsson; K E Kuettner; N Sorgente; V C Hascal
Journal:  Atherosclerosis       Date:  1975 Jul-Aug       Impact factor: 5.162

6.  Collagen-proteoglycan relationships in epiphyseal cartilage.

Authors:  R Eisenstein; S E Larsson; N Sorgente; K E Kuettner
Journal:  Am J Pathol       Date:  1973-11       Impact factor: 4.307

7.  Presence of heparan sulfate in the glomerular basement membrane.

Authors:  Y S Kanwar; M G Farquhar
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

8.  Immunochemical analysis of cartilage proteoglycans. Antigenic determinants of substructures.

Authors:  J Wieslander; D Heinegård
Journal:  Biochem J       Date:  1979-04-01       Impact factor: 3.857

9.  Role of molecular charge in glomerular permeability. Tracer studies with cationized ferritins.

Authors:  H G Rennke; R S Cotran; M A Venkatachalam
Journal:  J Cell Biol       Date:  1975-12       Impact factor: 10.539

10.  Distribution of a major connective tissue protein, fibronectin, in normal human tissues.

Authors:  S Stenman; A Vaheri
Journal:  J Exp Med       Date:  1978-04-01       Impact factor: 14.307
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  5 in total

1.  Glycoconjugates in normal human kidney. A histochemical study using 13 biotinylated lectins.

Authors:  L D Truong; V T Phung; Y Yoshikawa; C A Mattioli
Journal:  Histochemistry       Date:  1988

2.  Influence of cetylpyridinium chloride on the ultrastructural appearance of sulphated glycosaminoglycans in human colonic mucosa.

Authors:  F Malchiodi Albedi; A M Cassano; F Ciaralli; G Donelli; A Giuliani; P Mingazzini; V Marinozzi
Journal:  Histochemistry       Date:  1988

Review 3.  Antigens of the human glomerular basement membrane.

Authors:  L S Fouser; A F Michael
Journal:  Springer Semin Immunopathol       Date:  1987

4.  The glycosaminoglycan content of renal basement membranes in the congenital nephrotic syndrome of the Finnish type.

Authors:  L P Van den Heuvel; J Van den Born; H Jalanko; C H Schröder; J H Veerkamp; K J Assmann; J H Berden; C Holmberg; J Rapola; L A Monnens
Journal:  Pediatr Nephrol       Date:  1992-01       Impact factor: 3.714

5.  Quantitation of anionic sites in glomerular capillary basement membranes of Samoyed dogs with hereditary glomerulopathy.

Authors:  P S Thorner; B Jansen; J Liang; R Baumal; V Valli
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1987
  5 in total

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