Literature DB >> 6222035

Pool levels of UDP N-acetylglucosamine and UDP N-acetylglucosamine-enolpyruvate in Escherichia coli and correlation with peptidoglycan synthesis.

D Mengin-Lecreulx, B Flouret, J van Heijenoort.   

Abstract

A high-pressure liquid chromatography procedure was developed for the isolation and quantitation of UDP-N-acetylglucosamine, UDP-N-acetylglucosamine-enolpyruvate, and UDP-N-acetylmuramic acid, which are the early cytoplasmic precursors of bacterial peptidoglycan. In exponential-phase cells of Escherichia coli K-12, the intracellular concentration of UDP-N-acetylglucosamine was about 100 microM, whereas that of UDP-N-acetylglucosamine-enolpyruvate was only 2 microM. The phosphoenolpyruvate: UDP-N-acetylglucosamine transferase and UDP-N-acetylglucosamine-enolpyruvate reductase activities were investigated in extracts from E. coli. These activities appeared to be present in amounts sufficient for the ongoing rate of peptidoglycan synthesis. Certain uridine nucleotide peptidoglycan precursors were found to inhibit phosphoenolpyruvate: UDP-N-acetylglucosamine transferase activity.

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Year:  1983        PMID： 6222035      PMCID： PMC217602          DOI： 10.1128/jb.154.3.1284-1290.1983

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  19 in total

1.  Biosynthesis of uridine diphospho-N-acetylmuramic acid. 3. Purification and properties of uridine diphospho-N-acetylenolpyruvyl-glucosamine reductase.

Authors:  A Taku; K G Gunetileke; R A Anwar
Journal:  J Biol Chem       Date:  1970-10-10       Impact factor: 5.157

2.  Concentrations of nicotinamide nucleotide coenzymes in micro-organisms.

Authors:  J London; M Knight
Journal:  J Gen Microbiol       Date:  1966-08

3.  Biosynthesis of uridine diphospho-N-acetyl muramic acid.

Authors:  K G Gunetileke; R A Anwar
Journal:  J Biol Chem       Date:  1966-12-10       Impact factor: 5.157

4.  Inhibition of phosphoenolpyruvate:uridine diphosphate N-acetylglucosamine enolpyruvyltransferase by uridine diphosphate N-acetylmuramyl peptides.

Authors:  P S Venkateswaran; E J Lugtenberg; H C Wu
Journal:  Biochim Biophys Acta       Date:  1973-02-15

5.  The effect of carbon and nitrogen sources on the level of metabolic intermediates in Escherichia coli.

Authors:  O H Lowry; J Carter; J B Ward; L Glaser
Journal:  J Biol Chem       Date:  1971-11       Impact factor: 5.157

6.  Biosynthesis of uridine diphospho-N-acetylmuramic acid. IV. Activation of uridine diphospho-N-acetylenolpyruvylglucosamine reductase by monovalent cations.

Authors:  A Taku; R A Anwar
Journal:  J Biol Chem       Date:  1973-07-25       Impact factor: 5.157

7.  Partial purification and properties of the pyruvate-uridine diphospho-N-acetylglucosamine transferase from Staphylococcus epidermidis.

Authors:  G G Wickus; J L Strominger
Journal:  J Bacteriol       Date:  1973-01       Impact factor: 3.490

8.  Partial purification and some properties of the uridine diphospho-N-acetylglucosamine-enolpyruvate reductase from Staphylococcus epidermidis.

Authors:  G G Wickus; P A Rubenstein; A D Warth; J L Strominger
Journal:  J Bacteriol       Date:  1973-01       Impact factor: 3.490

9.  The control of synthesis of bacterial cell walls. Interaction in the synthesis of nucleotide precursors.

Authors:  R G Anderson; L J Douglas; H Hussey; J Baddiley
Journal:  Biochem J       Date:  1973-12       Impact factor: 3.857

10.  Biosynthesis of uridine diphospho-N-acetylmuramic acid. II. Purification and properties of pyruvate-uridine diphospho-N-acetylglucosamine transferase and characterization of uridine diphospho-N-acetylenopyruvylglucosamine.

Authors:  K G Gunetileke; R A Anwar
Journal:  J Biol Chem       Date:  1968-11-10       Impact factor: 5.157
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  29 in total

1.  A structural and functional analysis of the glycosyltransferase BshA from Staphylococcus aureus: Insights into the reaction mechanism and regulation of bacillithiol production.

Authors:  Christopher J Royer; Paul D Cook
Journal:  Protein Sci       Date:  2019-04-24       Impact factor: 6.725

2.  The murG gene of Escherichia coli codes for the UDP-N-acetylglucosamine: N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase involved in the membrane steps of peptidoglycan synthesis.

Authors:  D Mengin-Lecreulx; L Texier; M Rousseau; J van Heijenoort
Journal:  J Bacteriol       Date:  1991-08       Impact factor: 3.490

3.  The GTPase CpgA is implicated in the deposition of the peptidoglycan sacculus in Bacillus subtilis.

Authors:  Cédric Absalon; Kassem Hamze; Didier Blanot; Claude Frehel; Rut Carballido-Lopez; Barry I Holland; Jean van Heijenoort; Simone J Séror
Journal:  J Bacteriol       Date:  2008-03-14       Impact factor: 3.490

4.  Effect of slow growth on metabolism of Escherichia coli, as revealed by global metabolite pool ("metabolome") analysis.

Authors:  H Tweeddale; L Notley-McRobb; T Ferenci
Journal:  J Bacteriol       Date:  1998-10       Impact factor: 3.490

5.  Diaminopimelic Acid Amidation in Corynebacteriales: NEW INSIGHTS INTO THE ROLE OF LtsA IN PEPTIDOGLYCAN MODIFICATION.

Authors:  Marjorie Levefaudes; Delphine Patin; Célia de Sousa-d'Auria; Mohamed Chami; Didier Blanot; Mireille Hervé; Michel Arthur; Christine Houssin; Dominique Mengin-Lecreulx
Journal:  J Biol Chem       Date:  2015-04-06       Impact factor: 5.157

6.  Contribution of the Pmra promoter to expression of genes in the Escherichia coli mra cluster of cell envelope biosynthesis and cell division genes.

Authors:  D Mengin-Lecreulx; J Ayala; A Bouhss; J van Heijenoort; C Parquet; H Hara
Journal:  J Bacteriol       Date:  1998-09       Impact factor: 3.490

7.  Identification of the glmU gene encoding N-acetylglucosamine-1-phosphate uridyltransferase in Escherichia coli.

Authors:  D Mengin-Lecreulx; J van Heijenoort
Journal:  J Bacteriol       Date:  1993-10       Impact factor: 3.490

8.  Identification of the Escherichia coli murI gene, which is required for the biosynthesis of D-glutamic acid, a specific component of bacterial peptidoglycan.

Authors:  P Doublet; J van Heijenoort; D Mengin-Lecreulx
Journal:  J Bacteriol       Date:  1992-09       Impact factor: 3.490

9.  Copurification of glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase activities of Escherichia coli: characterization of the glmU gene product as a bifunctional enzyme catalyzing two subsequent steps in the pathway for UDP-N-acetylglucosamine synthesis.

Authors:  D Mengin-Lecreulx; J van Heijenoort
Journal:  J Bacteriol       Date:  1994-09       Impact factor: 3.490

10.  The murI gene of Escherichia coli is an essential gene that encodes a glutamate racemase activity.

Authors:  P Doublet; J van Heijenoort; J P Bohin; D Mengin-Lecreulx
Journal:  J Bacteriol       Date:  1993-05       Impact factor: 3.490
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