Chemical quality control of [99mTc]plasmin by affinityradiochromatography.

To establish a method for radiochemical quality control of [99mTc]plasmin based on the known affinity of plasmin for lysine residues, human [113Sn]plasmin and [99mTc]plasmin formed by different methods were analyzed in an affinityradiochromatographic system of lysine coupled to CNBr-activated sepharosis. From the observed immobilizations of the radioactivity when the plasmin was bound to the lysine-sepharosis, radiochemical purities of radiolabelled plasmin could be calculated. The effects of presence of Sn(II) and tartaric and gentisic acid and the remobilizations induced by 6-aminohexanoic acid were studied.