Literature DB >> 6219991

A kinetic mechanism for the poly(C)-dependent ATPase of the Escherichia coli transcription termination protein, rho.

J A Sharp, J L Galloway, T Platt.   

Abstract

A study of the initial velocity kinetics of the Escherichia coli transcription termination protein rho, with respect to its poly(C)-dependent ATPase, indicates that this reaction occurs by an ordered sequential mechanism. Product inhibition and substrate analogue studies suggest that ATP binding must precede the binding of poly(C) and that the order of release of the products is ADP followed by Pi, then poly(C). A possible mechanism for relating the ATPase to the termination reaction of rho is discussed in relation to the model for rho proposed by Richardson (5).

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Year:  1983        PMID： 6219991

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  6 in total

1.  Escherichia coli transcription termination factor rho has a two-domain structure in its activated form.

Authors:  D G Bear; C L Andrews; J D Singer; W D Morgan; R A Grant; P H von Hippel; T Platt
Journal:  Proc Natl Acad Sci U S A       Date:  1985-04       Impact factor: 11.205

2.  The nucleotide sequence of the rho gene of E. coli K-12.

Authors:  J L Pinkham; T Platt
Journal:  Nucleic Acids Res       Date:  1983-06-11       Impact factor: 16.971

3.  Binding and translocation of termination factor rho studied at the single-molecule level.

Authors:  Daniel J Koslover; Furqan M Fazal; Rachel A Mooney; Robert Landick; Steven M Block
Journal:  J Mol Biol       Date:  2012-08-09       Impact factor: 5.469

4.  Maximizing gene expression from plasmid vectors containing the lambda PL promoter: strategies for overproducing transcription termination factor rho.

Authors:  J E Mott; R A Grant; Y S Ho; T Platt
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

5.  Structure of rho factor: an RNA-binding domain and a separate region with strong similarity to proven ATP-binding domains.

Authors:  A J Dombroski; T Platt
Journal:  Proc Natl Acad Sci U S A       Date:  1988-04       Impact factor: 11.205

6.  Maturation of Escherichia coli tryptophan operon mRNA: evidence for 3' exonucleolytic processing after rho-dependent termination.

Authors:  J E Mott; J L Galloway; T Platt
Journal:  EMBO J       Date:  1985-07       Impact factor: 11.598
  6 in total

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