Literature DB >> 6219006

Evidence for the presence of DNase-actin complex in L1210 leukemia cells.

M Malicka-Blaszkiewicz, J S Roth.   

Abstract

L1210 leukemia cell cytosol was analysed for the presence of DNase I activity. No free activity was determined in crude cytosol. DNase I enzyme was found to occur in a latent form bound to cytoplasmic actin. DNase-actin complex was partially isolated by Sephadex filtration and DNase I-like activity was demonstrated after SDS gel electrophoresis of the complex and enzyme renaturation. The results were compared with those for synthetic complex of pancreatic bovine DNase I and chicken muscle actin.

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Year:  1983        PMID： 6219006     DOI： 10.1016/0014-5793(83)80155-0

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  3 in total

1.  Localization of deoxyribonuclease I gene transcripts and protein in rat tissues and its correlation with apoptotic cell elimination.

Authors:  S Zanotti; B Polzar; H Stephan; U Doll; J Niessing; H G Mannherz
Journal:  Histochem Cell Biol       Date:  1995-05       Impact factor: 4.304

2.  Androgen ablation leads to an upregulation and intranuclear accumulation of deoxyribonuclease I in rat prostate epithelial cells paralleling their apoptotic elimination.

Authors:  F Rauch; B Polzar; H Stephan; S Zanotti; R Paddenberg; H G Mannherz
Journal:  J Cell Biol       Date:  1997-05-19       Impact factor: 10.539

3.  Characterization of the endogenous deoxyribonuclease involved in nuclear DNA degradation during apoptosis (programmed cell death).

Authors:  M C Peitsch; B Polzar; H Stephan; T Crompton; H R MacDonald; H G Mannherz; J Tschopp
Journal:  EMBO J       Date:  1993-01       Impact factor: 11.598
  3 in total

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