Literature DB >> 6218207

Interaction of staphylococcal protein A in enzyme-linked immunosorbent assays for detecting staphylococcal antigens.

S Notermans, P Timmermans, J Nagel.   

Abstract

ELISA procedures for detecting staphylococcal antigens may be subject to interference by reactions between staphylococcal protein A (SPA) and IgG molecules. It was found that rabbit IgG reacted with SPA, both in the native state and after conjugation with peroxidase. Sheep IgG, however, did not react with SPA if conjugated with peroxidase. Peroxidase conjugated SPA reacted with rabbit IgG but not with sheep IgG. These results demonstrate that the source of IgG used in an ELISA system is of major importance to correct quantitation of staphylococcal antigens.

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Year:  1982        PMID： 6218207     DOI： 10.1016/0022-1759(82)90074-6

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  10 in total

1.  Development of an immunochromatographic strip for simple detection of penicillin-binding protein 2'.

Authors:  Hidehito Matsui; Hideaki Hanaki; Megumi Inoue; Hiroyuki Akama; Taiji Nakae; Keisuke Sunakawa; Satoshi Omura
Journal:  Clin Vaccine Immunol       Date:  2010-12-22

2.  Production of enterotoxin A by supposedly nonenterotoxigenic Staphylococcus aureus strains.

Authors:  E Gomez-Lucía; J Goyache; J A Orden; J L Blanco; J A Ruiz-Santa-Quiteria; L Domínguez; G Suárez
Journal:  Appl Environ Microbiol       Date:  1989-06       Impact factor: 4.792

3.  Competitive, enzyme-linked immunosorbent assay for toxic shock syndrome toxin 1.

Authors:  J Parsonnet; J T Mills; Z A Gillis; G B Pier
Journal:  J Clin Microbiol       Date:  1985-07       Impact factor: 5.948

4.  The use of a sandwich ELISA for the detection of staphylococcal enterotoxin A in foods from outbreaks of food poisoning.

Authors:  A A Wieneke; R J Gilbert
Journal:  J Hyg (Lond)       Date:  1985-08

5.  Comparative evaluation of different enzyme-linked immunosorbent assay systems for the detection of staphylococcal enterotoxins A, B, C, and D.

Authors:  H Fey; H Pfister; O Rüegg
Journal:  J Clin Microbiol       Date:  1984-01       Impact factor: 5.948

Review 6.  Clinical, microbial, and biochemical aspects of the exfoliative toxins causing staphylococcal scalded-skin syndrome.

Authors:  S Ladhani; C L Joannou; D P Lochrie; R W Evans; S M Poston
Journal:  Clin Microbiol Rev       Date:  1999-04       Impact factor: 26.132

7.  Enzyme immunoassay for the detection of group A streptococcal antigen.

Authors:  K M Knigge; J L Babb; J R Firca; K Ancell; T G Bloomster; B A Marchlewicz
Journal:  J Clin Microbiol       Date:  1984-10       Impact factor: 5.948

8.  Colony immunoblot assay for the detection of staphylococcal toxic shock syndrome toxin 1 (TSST-1) with anti-TSST-1 F(ab')2 fragments.

Authors:  R H See; S Adilman; K H Bartlett; A W Chow
Journal:  J Clin Microbiol       Date:  1989-09       Impact factor: 5.948

9.  Quantitative determination of staphylococcal enterotoxin A by an enzyme-linked immunosorbent assay using a combination of polyclonal and monoclonal antibodies and biotin-streptavidin interaction.

Authors:  C Edwin
Journal:  J Clin Microbiol       Date:  1989-07       Impact factor: 5.948

10.  Stool desorbing activity: a possible cause of false-positive reactions in competitive enzyme immunoassays.

Authors:  M Hanvanich; R Viscidi; B E Laughon; J G Bartlett; R H Yolken
Journal:  J Clin Microbiol       Date:  1985-02       Impact factor: 5.948
  10 in total

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