Literature DB >> 6218161

Molecular weight and subunit stoichiometry of the chloroplast coupling factor 1 from Chlamydomonas reinhardi.

S Merchant, S L Shaner, B R Selman.   

Abstract

The molecular weight of the Chlamydomonas reinhardi coupling factor 1 (CF1) is 4.2 X 10(5) as determined by gel exclusion chromatography and sedimentation equilibrium. In addition, a measured sedimentation coefficient of 12.9 S results in a calculated molecular weight of 3.9 X 10(5). These molecular weight estimates are too high to support an alpha 2 beta 2-type subunit stoichiometry and are suggestive of an alpha 3 beta 3-type enzyme. The subunit stoichiometry of the C. reinhardi CF1 was determined from the distribution of label into the subunits of uniformly labeled CF1. An alpha: beta: gamma: epsilon ration of 2.9:2.9:1:1 was obtained.

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Year:  1983        PMID： 6218161

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  7 in total

1.  Cooperative transient trapping of photosystem II protons by the integral membrane portion (CF0) of chloroplast ATP-synthase after mild extraction of the four-subunit catalytic part (CF1).

Authors:  W Junge; Y Q Hong; L P Qian; A Viale
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205

2.  Profile of Sabeeha Merchant.

Authors:  Tinsley H Davis
Journal:  Proc Natl Acad Sci U S A       Date:  2015-02-09       Impact factor: 11.205

3.  Photosynthetic ATPases: purification, properties, subunit isolation and function.

Authors:  S Merchant; B R Selman
Journal:  Photosynth Res       Date:  1985-03       Impact factor: 3.573

Review 4.  New molecular aspects of energy-transducing protein complexes.

Authors:  N Nelson; S Cidon
Journal:  J Bioenerg Biomembr       Date:  1984-02       Impact factor: 2.945

Review 5.  Recent developments on structural and functional aspects of the F1 sector of H+-linked ATPases.

Authors:  P V Vignais; M Satre
Journal:  Mol Cell Biochem       Date:  1984       Impact factor: 3.396

6.  Isolation of a cDNA clone for the gamma subunit of the chloroplast ATP synthase of Chlamydomonas reinhardtii: import and cleavage of the precursor protein.

Authors:  L M Yu; S Merchant; S M Theg; B R Selman
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205

7.  Plastome mutation affecting the chloroplast ATP synthase involves a post-transcriptional defect.

Authors:  B B Sears; R G Herrmann
Journal:  Curr Genet       Date:  1985       Impact factor: 3.886
  7 in total

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