Literature DB >> 6217813

Large-scale isolation of human erythrocyte Ca2+-transport ATPase.

K Gietzen, J Kolandt.   

Abstract

A rapid procedure for preparing large quantities of purified erythrocyte Ca2+-transport ATPase is presented. The method involves: (1) fast preparation of calmodulin-deficient, essentially haemoglobin-free, erythrocyte membranes by molecular filtration using Pellicon filters; (2) solubilization of membrane proteins by deoxycholate; and (3) a batch procedure using calmodulin-Sepharose 4B gel for purification of Ca2+-transport ATPase.

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Year:  1982        PMID： 6217813      PMCID： PMC1153836          DOI： 10.1042/bj2070155

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  14 in total

1.  Enhancement of (Ca2+ + Mg2+)-ATPase activity of human erythrocyte membranes by hemolysis in isosmotic imidazole buffer. I. General properties of variously prepared membranes and the mechanism of the isosmotic imidazole effect.

Authors:  M L Farrance; F F Vincenzi
Journal:  Biochim Biophys Acta       Date:  1977-11-15

2.  Calmodulin. Development and application of a sensitive radioimmunoassay.

Authors:  J G Chafouleas; J R Dedman; R P Munjaal; A R Means
Journal:  J Biol Chem       Date:  1979-10-25       Impact factor: 5.157

3.  Affinity chromatography purification of a cyclic nucleotide phosphodiesterase using immobilized modulator protein, a troponin C-like protein from brain.

Authors:  D M Watterson; T C Vanaman
Journal:  Biochem Biophys Res Commun       Date:  1976-11-08       Impact factor: 3.575

4.  Dependence on calcium concentration and stoichiometry of the calcium pump in human red cells.

Authors:  H J Schatzmann
Journal:  J Physiol       Date:  1973-12       Impact factor: 5.182

5.  Isolation of sarcoplasmic reticulum by zonal centrifugation and purification of Ca 2+ -pump and Ca 2+ -binding proteins.

Authors:  G Meissner; G E Conner; S Fleischer
Journal:  Biochim Biophys Acta       Date:  1973-03-16

6.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

7.  Reconstitution of the Ca2+-transport system of human erythrocytes.

Authors:  K Gietzen; S Seiler; S Fleischer; H U Wolf
Journal:  Biochem J       Date:  1980-04-15       Impact factor: 3.857

8.  Maturation of the head of bacteriophage T4. I. DNA packaging events.

Authors:  U K Laemmli; M Favre
Journal:  J Mol Biol       Date:  1973-11-15       Impact factor: 5.469

9.  Large scale isolation of human erythrocyte membranes by high volume molecular filtration.

Authors:  T L Rosenberry; J F Chen; M M Lee; T A Moulton; P Onigman
Journal:  J Biochem Biophys Methods       Date:  1981-01

10.  Calmodulin affinity chromatography yields a functional purified erythrocyte (Ca+ + Mg2+)-dependent adenosine triphosphatase.

Authors:  K Gietzen; M Tejcka; H U Wolf
Journal:  Biochem J       Date:  1980-07-01       Impact factor: 3.857
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  6 in total

1.  Smooth muscle expresses a cardiac/slow muscle isoform of the Ca2+-transport ATPase in its endoplasmic reticulum.

Authors:  F Wuytack; Y Kanmura; J A Eggermont; L Raeymaekers; J Verbist; D Hartweg; K Gietzen; R Casteels
Journal:  Biochem J       Date:  1989-01-01       Impact factor: 3.857

2.  Antibodies against erythrocyte Ca2+-transport ATPase specifically inhibit the calmodulin-dependent fraction of the enzyme's activity.

Authors:  K Gietzen; J Kolandt
Journal:  Biochem J       Date:  1985-06-01       Impact factor: 3.857

3.  Comparison of the calmodulin antagonists compound 48/80 and calmidazolium.

Authors:  K Gietzen
Journal:  Biochem J       Date:  1983-12-15       Impact factor: 3.857

4.  Abundance of the Ca2+-pumping ATPase in pig erythrocyte membranes.

Authors:  C O Bewaji; E A Bababunmi
Journal:  Biochem J       Date:  1987-11-15       Impact factor: 3.857

5.  Cyclic GMP-dependent protein kinase stimulates the plasmalemmal Ca2+ pump of smooth muscle via phosphorylation of phosphatidylinositol.

Authors:  M Vrolix; L Raeymaekers; F Wuytack; F Hofmann; R Casteels
Journal:  Biochem J       Date:  1988-11-01       Impact factor: 3.857

6.  Irreversible effects of calcium ions on the plasma membrane calcium pump.

Authors:  D G Ward; T J Walton; J D Cavieres
Journal:  J Membr Biol       Date:  1993-12       Impact factor: 1.843
  6 in total

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