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Literature DB >> 6214548

Application of hybrid plasmids carrying glycolysis genes to ATP production by Escherichia coli.

M Shimosaka, Y Fukuda, K Murata, A Kimura.

Abstract

The closely linked structural genes of phosphofructokinase (pfkA) and triosephosphate isomerase (tpi) of Escherichia coli were separately cloned onto plasmid pBR322. By gene dosage effects, transformed cells of E. coli C600 with these pBR322 hybrid plasmids showed 7- and 16-fold increases in the specific activities of phosphofructokinase and triosephosphate isomerase, respectively, over the specific activities in C600. Dried preparations of E. coli cells dosed with these genes showed appreciably high ATP-regenerating activity.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1982 PMID： 6214548 PMCID： PMC221379 DOI： 10.1128/jb.152.1.98-103.1982

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

13 in total

1. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors: B J DAVIS
Journal: Ann N Y Acad Sci Date: 1964-12-28 Impact factor: 5.691

2. E. coli phosphofructokinase synthesized in vitro from a ColE1 hybrid plasmid.

Authors: J A Thomson
Journal: Gene Date: 1977-07 Impact factor: 3.688

3. Deletion mapping of the polA-metB region of the Escherichia coli chromosome.

Authors: G Pahel; F R Bloom; B Tyler
Journal: J Bacteriol Date: 1979-05 Impact factor: 3.490

4. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.

Authors: R B Helling; H M Goodman; H W Boyer
Journal: J Virol Date: 1974-11 Impact factor: 5.103

5. Measurement of the intermediates of the photosynthetic carbon reduction cycle, using enzymatic methods.

Authors: E Latzko; M Gibbs
Journal: Methods Enzymol Date: 1972 Impact factor: 1.600

6. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

Authors: L Clarke; J Carbon
Journal: Cell Date: 1976-09 Impact factor: 41.582

7. Phosphorylation of mononucleotides and formation of cytidine 5'-diphosphate-choline and sugar nucleotides by respiration-deficient mutants of yeasts.

Authors: A Kimura; K Hirose; Y Kariya; S Nagai
Journal: J Bacteriol Date: 1976-02 Impact factor: 3.490

8. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.

Authors: J A Meyers; D Sanchez; L P Elwell; S Falkow
Journal: J Bacteriol Date: 1976-09 Impact factor: 3.490

Application of hybrid plasmids carrying glycolysis genes to ATP production by Escherichia coli.

1. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

2. E. coli phosphofructokinase synthesized in vitro from a ColE1 hybrid plasmid.

3. Deletion mapping of the polA-metB region of the Escherichia coli chromosome.

4. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.

5. Measurement of the intermediates of the photosynthetic carbon reduction cycle, using enzymatic methods.

6. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

7. Phosphorylation of mononucleotides and formation of cytidine 5'-diphosphate-choline and sugar nucleotides by respiration-deficient mutants of yeasts.

8. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.

9. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

10. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

1. Nucleotide sequence of the triose phosphate isomerase gene of Escherichia coli.

2. Physical and genetic structure of the glpK-cpxA interval of the Escherichia coli K-12 chromosome.

3. Expression of the Escherichia coli pfkA gene in Alcaligenes eutrophus and in other gram-negative bacteria.

4. Isolation of superoxide dismutase mutants in Escherichia coli: is superoxide dismutase necessary for aerobic life?