The effect of hypoxia on the refractoriness of the canine ventricular muscle.

The effect of hypoxia on the relationship between refractoriness and transmembrane action potential was studied in isolated canine papillary muscle preparations. Test stimuli were applied at variable intervals after every 10 to 15 driving stimuli, and action potentials were recorded through an intracellular microelectrode located near the stimulus site. During hypoxia of 30 to 90 min duration, induced by nitrogenization of the perfusate, action potential duration (APD) was tremendously decreased in association with decline in the amplitude and rising velocity. The resting potential was also reduced. Concurrently the effective refractory period (ERP) was decreased, but its decrement was smaller than that of the APD. The total refractory period (TRP) was, on the other hand, prolonged. In consequence, not only the TRP but the ERP as well fairly outlasted full repolarization as a result of the hypoxia. The duration of the relative refractory period (RRP) was increased during hypoxia as the result of the changes in ERP and TRP, and graded local responses were registered frequently in the early phase of RRP. These facts were thought to be related to the genesis of arrhythmias in the ischemic heart.