Production of a hybridoma T suppressor cell and suppressor factor.

Two hybridoma T-cell lines were prepared by fusion of burro erythrocyte (BRBC)--specific T suppressor cells with the BW 5147 thymoma line. Culture supernatants from these T cell hybrids specifically suppressed the in vitro IgG antibody response to BRBC. Clones P3E8 and P4B10 were both removed via affinity columns bearing antibodies to BRBC and antibodies to I-Jk subregion determinants. These results indicated that the hybridoma factors were I-Jk determinant bearing and anti-idiotype determinant bearing. P3E8 supernatant was major histocompatibility complex (MHC) restricted, suppressing only H-2k haplotype spleen cells. Clone P4B10 supernatant was not MHC restricted, suppressing k, s, b, and d haplotype spleen cells. The properties of these two hybridoma factors would indicate they are derived from Ts2 cells.