Literature DB >> 6212585

Purification and properties of a transcriptional activator. The cII protein of phage lambda.

Y Ho, M Lewis, M Rosenberg.   

Abstract

We have purified the phage lambda transcriptional activator protein cII. The procedure described allows cII to be obtained in both high purity and yield, and thus allows detailed physical and chemical analysis. We demonstrate that cII in solution is a tetrameric protein and that it undergoes specific processing at its NH2-terminal end. In addition, the protein is characterized as to its molar extinction coefficient, molecular weight, amino acid composition, isoelectric point, alpha-helical content, and antigenic capability.

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Year:  1982        PMID： 6212585

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  17 in total

Review 1.  Regulation by proteolysis: energy-dependent proteases and their targets.

Authors:  S Gottesman; M R Maurizi
Journal:  Microbiol Rev       Date:  1992-12

2.  Interactions among CII protein, RNA polymerase and the lambda PRE promoter: contacts between RNA polymerase and the -35 region of PRE are identical in the presence and absence of CII protein.

Authors:  Michael T Marr; Jeffrey W Roberts; Susan E Brown; Matthew Klee; Gary N Gussin
Journal:  Nucleic Acids Res       Date:  2004-02-10       Impact factor: 16.971

3.  Overproduction and purification of the luxR gene product: Transcriptional activator of the Vibrio fischeri luminescence system.

Authors:  H B Kaplan; E P Greenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1987-10       Impact factor: 11.205

4.  Structure of lambda CII: implications for recognition of direct-repeat DNA by an unusual tetrameric organization.

Authors:  Ajit B Datta; Santosh Panjikar; Manfred S Weiss; Pinak Chakrabarti; Pradeep Parrack
Journal:  Proc Natl Acad Sci U S A       Date:  2005-08-01       Impact factor: 11.205

5.  Synchronizing genetic relaxation oscillators by intercell signaling.

Authors:  David McMillen; Nancy Kopell; Jeff Hasty; J J Collins
Journal:  Proc Natl Acad Sci U S A       Date:  2002-01-22       Impact factor: 11.205

6.  Host regulation of lysogenic decision in bacteriophage lambda: transmembrane modulation of FtsH (HflB), the cII degrading protease, by HflKC (HflA).

Authors:  A Kihara; Y Akiyama; K Ito
Journal:  Proc Natl Acad Sci U S A       Date:  1997-05-27       Impact factor: 11.205

7.  A cII-dependent promoter is located within the Q gene of bacteriophage lambda.

Authors:  B C Hoopes; W R McClure
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

8.  Cleavage of the cII protein of phage lambda by purified HflA protease: control of the switch between lysis and lysogeny.

Authors:  H H Cheng; P J Muhlrad; M A Hoyt; H Echols
Journal:  Proc Natl Acad Sci U S A       Date:  1988-11       Impact factor: 11.205

9.  Kinetic analysis of mutations affecting the cII activation site at the PRE promoter of bacteriophage lambda.

Authors:  M C Shih; G N Gussin
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

10.  Efficient expression of influenza virus NS1 nonstructural proteins in Escherichia coli.

Authors:  J F Young; U Desselberger; P Palese; B Ferguson; A R Shatzman; M Rosenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1983-10       Impact factor: 11.205
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