Literature DB >> 6211442

Direct fluorescence measurements of Mg2+ binding to sarcoplasmic reticulum ATPase.

F Guillain, M P Gingold, P Champeil.   

Abstract

In the absence of calcium, interaction of magnesium with SR-ATPase induced a blue shift in intrinsic fluorescence emission. This Mg2+-induced fluorescence change was pH-dependent and an apparent Mg dissociation constant of 5 mM was found at pH 7. Equilibrium studies showed that magnesium competes for the high affinity Ca2+ binding sites and stopped flow measurements of the transient kinetics indicated a multistep interaction between magnesium and the calcium pump. These results suggest that magnesium drives the sarcoplasmic reticulum atpase toward an E.Mg species which might be a dead-end complex.

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Year:  1982        PMID： 6211442

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  10 in total

1.  Thermodynamics of Cation Binding to the Sarcoendoplasmic Reticulum Calcium ATPase Pump and Impacts on Enzyme Function.

Authors:  Bin Sun; Bradley D Stewart; Amir N Kucharski; Peter M Kekenes-Huskey
Journal:  J Chem Theory Comput       Date:  2019-03-13       Impact factor: 6.006

2.  Mechanism of inhibition of Ca(2+)-ATPase by myotoxin a.

Authors:  K J Baker; J M East; A G Lee
Journal:  Biochem J       Date:  1995-04-15       Impact factor: 3.857

3.  Characterization of calcium, nucleotide, phosphate, and vanadate bound states by derivatization of sarcoplasmic reticulum ATPase with ThioGlo1.

Authors:  S Hua; D Fabris; G Inesi
Journal:  Biophys J       Date:  1999-10       Impact factor: 4.033

4.  Characterization of the single Ca(2+)-binding site on the Ca(2+)-ATPase reconstituted with short- or long-chain phosphatidylcholines.

Authors:  A P Starling; Y M Khan; J M East; A G Lee
Journal:  Biochem J       Date:  1994-12-01       Impact factor: 3.857

5.  Characterization of ruthenium red-binding sites of the Ca(2+)-ATPase from sarcoplasmic reticulum and their interaction with Ca(2+)-binding sites.

Authors:  S Corbalan-Garcia; J A Teruel; J C Gomez-Fernandez
Journal:  Biochem J       Date:  1992-11-01       Impact factor: 3.857

Review 6.  Energy turnover for Ca2+ cycling in skeletal muscle.

Authors:  C J Barclay; R C Woledge; N A Curtin
Journal:  J Muscle Res Cell Motil       Date:  2007-09-20       Impact factor: 2.698

7.  Effects of K+ on the binding of Ca2+ to the Ca(2+)-ATPase of sarcoplasmic reticulum.

Authors:  A G Lee; K Baker; Y M Khan; J M East
Journal:  Biochem J       Date:  1995-01-01       Impact factor: 3.857

8.  Binding of Ca2+ to the (Ca(2+)-Mg2+)-ATPase of sarcoplasmic reticulum: equilibrium studies.

Authors:  I M Henderson; Y M Khan; J M East; A G Lee
Journal:  Biochem J       Date:  1994-02-01       Impact factor: 3.857

9.  High-yield heterologous expression of wild type and mutant Ca(2+) ATPase: Characterization of Ca(2+) binding sites by charge transfer.

Authors:  Yueyong Liu; Rajendra Pilankatta; David Lewis; Giuseppe Inesi; Francesco Tadini-Buoninsegni; Gianluca Bartolommei; Maria Rosa Moncelli
Journal:  J Mol Biol       Date:  2009-06-24       Impact factor: 5.469

10.  Microsecond molecular dynamics simulations of Mg²⁺- and K⁺-bound E1 intermediate states of the calcium pump.

Authors:  L Michel Espinoza-Fonseca; Joseph M Autry; David D Thomas
Journal:  PLoS One       Date:  2014-04-23       Impact factor: 3.240
  10 in total

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