Literature DB >> 6210111

Uridine uptake by isolated intestinal epithelial cells of guinea pig.

M Schwenk, E Hegazy, V Lopez del Pino.   

Abstract

Uptake of uridine was studied in isolated intestinal epithelial cells of guinea pig. Uptake was not severely influenced by metabolism. Free uridine was accumulated within cells 13-fold. Uptake was saturable with an apparent Km value of 46 microM and a Vmax of 0.9 nmol/mg protein per min. Uracil inhibited uptake only slightly; adenosine, guanosine and cytosine inhibited strongly. Antimycin A and ouabain inhibited almost 90%. If the extracellular Na+ concentration was decreased to 5 mM, the rate of uptake decreased 6.5-fold. The stimulatory effect of Na+ was related to the transmembraneous Na+-gradient. Cells from jejunum transported about 30% faster than cells from ileum. In conclusion, isolated enterocytes of guinea pig posses an active transport system for uridine.

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Year:  1984        PMID： 6210111     DOI： 10.1016/0167-4889(84)90020-x

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  14 in total

Review 1.  Membrane transport and the antineoplastic action of nucleoside analogues.

Authors:  F M Sirotnak; J R Barrueco
Journal:  Cancer Metastasis Rev       Date:  1987       Impact factor: 9.264

2.  Multiple sodium-dependent nucleoside transport systems in bovine renal brush-border membrane vesicles.

Authors:  T C Williams; S M Jarvis
Journal:  Biochem J       Date:  1991-02-15       Impact factor: 3.857

3.  Induction of the differentiation of HL-60 cells by phorbol 12-myristate 13-acetate activates a Na(+)-dependent uridine-transport system. Involvement of protein kinase C.

Authors:  C W Lee; J A Sokoloski; A C Sartorelli; R E Handschumacher
Journal:  Biochem J       Date:  1991-02-15       Impact factor: 3.857

Review 4.  The concentrative nucleoside transporter family, SLC28.

Authors:  Jennifer H Gray; Ryan P Owen; Kathleen M Giacomini
Journal:  Pflugers Arch       Date:  2003-07-11       Impact factor: 3.657

5.  Expression of high levels of nitrobenzylthioinosine-sensitive nucleoside transport in cultured human choriocarcinoma (BeWo) cells.

Authors:  C E Boumah; D L Hogue; C E Cass
Journal:  Biochem J       Date:  1992-12-15       Impact factor: 3.857

6.  The transport and metabolism of naturally occurring pyrimidine nucleosides by isolated rat jejunum.

Authors:  J R Bronk; J G Hastewell
Journal:  J Physiol       Date:  1988-01       Impact factor: 5.182

7.  Enhanced delivery to target cells by heat-sensitive immunoliposomes.

Authors:  S M Sullivan; L Huang
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205

8.  Expression of the rabbit intestinal N2 Na+/nucleoside transporter in Xenopus laevis oocytes.

Authors:  S M Jarvis; D A Griffith
Journal:  Biochem J       Date:  1991-09-01       Impact factor: 3.857

9.  Residual nitrobenzylthioinosine-resistant nucleoside transport in a transport mutant (AE1) of S49 murine T-lymphoma cells.

Authors:  P G Plagemann; C Woffendin
Journal:  Mol Cell Biol       Date:  1987-01       Impact factor: 4.272

10.  Incomplete nucleoside transport deficiency with increased hypoxanthine transport capability in mutant T-lymphoblastoid cells.

Authors:  B Aronow; P Hollingsworth; J Patrick; B Ullman
Journal:  Mol Cell Biol       Date:  1986-04       Impact factor: 4.272
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