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Literature DB >> 6209333

The development and performance of a simple, sensitive method for the detection of Cryptosporidium oocysts in faeces.

Abstract

Features are described of a new staining method for the detection of Cryptosporidium oocysts in faeces. Smears, fixed in acid-methanol, are stained with heated safranin and counterstained with methylene blue. Oocysts stain a vivid orange-pink and are easily recognized. The method is rapid and simple with little source of error. The method is more sensitive than currently recommended Ziehl-Neelson methods; of 26 cases of cryptosporidiosis diagnosed by the new method only 19 were detected by acid-fast and only 11 by acid-alcohol-fast methods. Oocysts can be stained by safranin-methylene blue after concentration by various methods, in paraffin-embedded material, and after storage for months at various temperatures.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1984 PMID： 6209333 PMCID： PMC2129424 DOI： 10.1017/s0022172400064858

Source DB: PubMed Journal: J Hyg (Lond) ISSN： 0022-1724

15 in total

1. Staining of faecal yeasts and cryptosporidium oocysts.

Authors: K W Angus; I Campbell; E W Gray; D Sherwood
Journal: Vet Rec Date: 1981-02-21 Impact factor: 2.695

2. Staining of cryptosporidia by a modified Ziehl-Neelsen technique.

Authors: S A Henriksen; J F Pohlenz
Journal: Acta Vet Scand Date: 1981 Impact factor: 1.695

Review 3. Cryptosporidiosis in animals and humans.

Authors: S Tzipori
Journal: Microbiol Rev Date: 1983-03

4. Emerging zoonoses.

Authors: M G Schultz
Journal: N Engl J Med Date: 1983-05-26 Impact factor: 91.245

5. Vomiting and diarrhea associated with cryptosporidial infection.

Authors: S Tzipori; K W Angus; E W Gray; I Campbell
Journal: N Engl J Med Date: 1980-10-02 Impact factor: 91.245

6. Life-threatening diarrhea caused by Cryptosporidium in a child undergoing therapy for acute lymphocytic leukemia.

Authors: R A Miller; R E Holmberg; C R Clausen
Journal: J Pediatr Date: 1983-08 Impact factor: 4.406

7. Techniques for the recovery and identification of Cryptosporidium oocysts from stool specimens.

Authors: L S Garcia; D A Bruckner; T C Brewer; R Y Shimizu
Journal: J Clin Microbiol Date: 1983-07 Impact factor: 5.948

8. Three-step stool examination for cryptosporidiosis in 10 homosexual men with protracted watery diarrhea.

Authors: P Ma; R Soave
Journal: J Infect Dis Date: 1983-05 Impact factor: 5.226

9. Human cryptosporidiosis in immunocompetent and immunodeficient persons. Studies of an outbreak and experimental transmission.

Authors: W L Current; N C Reese; J V Ernst; W S Bailey; M B Heyman; W M Weinstein
Journal: N Engl J Med Date: 1983-05-26 Impact factor: 91.245

Review 9. Cryptosporidiosis.

Authors: W L Current; L S Garcia
Journal: Clin Microbiol Rev Date: 1991-07 Impact factor: 26.132

10. Serum immunoglobulin G, M and A response to Cryptosporidium parvum in Cryptosporidium-HIV co-infected patients.

Authors: Kirti Kaushik; Sumeeta Khurana; Ajay Wanchu; Nancy Malla
Journal: BMC Infect Dis Date: 2009-11-18 Impact factor: 3.090

The development and performance of a simple, sensitive method for the detection of Cryptosporidium oocysts in faeces.

1. Staining of faecal yeasts and cryptosporidium oocysts.

2. Staining of cryptosporidia by a modified Ziehl-Neelsen technique.

Review 3. Cryptosporidiosis in animals and humans.

4. Emerging zoonoses.

5. Vomiting and diarrhea associated with cryptosporidial infection.

6. Life-threatening diarrhea caused by Cryptosporidium in a child undergoing therapy for acute lymphocytic leukemia.

7. Techniques for the recovery and identification of Cryptosporidium oocysts from stool specimens.

8. Three-step stool examination for cryptosporidiosis in 10 homosexual men with protracted watery diarrhea.

9. Human cryptosporidiosis in immunocompetent and immunodeficient persons. Studies of an outbreak and experimental transmission.

Review 10. Cryptosporidiosis in man, domestic animals and birds: a review.

1. ACP Broadsheet 128: June 1991. Laboratory methods for diagnosing cryptosporidiosis.

Review 2. Epidemiological aspects of human cryptosporidiosis.

3. Uniform staining of Cyclospora oocysts in fecal smears by a modified safranin technique with microwave heating.

4. Cryptosporidium causing chronic diarrhea.

5. The incidence of cryptosporidiosis in children: a one-year prospective survey in a general hospital in Spain.

Review 6. Laboratory Diagnosis of Parasites from the Gastrointestinal Tract.

7. Rapid immunoassay for detection of Cryptosporidium oocysts.

8. Screening for cryptosporidial infection.

Review 9. Cryptosporidiosis.

10. Serum immunoglobulin G, M and A response to Cryptosporidium parvum in Cryptosporidium-HIV co-infected patients.