Literature DB >> 6207660

Immunoelectron microscopy of amylase in the human parotid gland. Ultrastructural localization by use of both the protein A-gold and the biotin-avidin-gold technique.

K P Zimmer, J Caselitz, G Seifert, G Grenner.   

Abstract

Specimens of the human parotid gland were studied by immuno-electron microscopy for the presence of amylase. Both the protein A-gold technique and the biotin-avidin-gold technique were used on the same specimens. Different fixations were tried. Amylase was detected in the zymogen granules in high amounts. This enzyme could even be seen in glutaraldehyde fixed and routinely embedded material. The subcellular localization of this enzyme opens a new field of functional morphological studies and studies in special tumours including acinic cell carcinomas.

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Year:  1984        PMID： 6207660     DOI： 10.1007/bf00704063

Source DB:  PubMed          Journal:  Virchows Arch A Pathol Anat Histopathol        ISSN： 0174-7398


  29 in total

1.  The avidin-biotin complex in affinity cytochemistry.

Authors:  E A Bayer; E Skutelsky; M Wilchek
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

2.  [Acinic cell adenocarcinoma of the salivary glands. Report of 4 cases with electron microscopic study of 2 tumors].

Authors:  R Hirtzler; B Oberman; M Kulis; N Ljubesić
Journal:  Arch Klin Exp Ohren Nasen Kehlkopfheilkd       Date:  1969

3.  The starch substrate film method for the localization of amylase activity. Suggestions for a standardized procedure.

Authors:  R J Smith; J Frommer
Journal:  J Histochem Cytochem       Date:  1973-02       Impact factor: 2.479

4.  Preservation of the secretory granules of rat parotid gland for electron microscopy.

Authors:  M R Robinovitch; L M Sreebny; E A Smuckler
Journal:  Exp Cell Res       Date:  1966-06       Impact factor: 3.905

5.  Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.

Authors:  S M Hsu; L Raine; H Fanger
Journal:  J Histochem Cytochem       Date:  1981-04       Impact factor: 2.479

6.  Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.

Authors:  G S Wood; R Warnke
Journal:  J Histochem Cytochem       Date:  1981-10       Impact factor: 2.479

7.  Morphology and cytochemistry of acinar secretory granules in normal and isoproterenol-treated rat submandibular glands.

Authors:  J A Simson; R M Dom; P L Sannes; S S Spicer
Journal:  J Microsc       Date:  1978-07       Impact factor: 1.758

8.  Use of colloidal gold particles in double-labeling immunoelectron microscopy of ultrathin frozen tissue sections.

Authors:  H J Geuze; J W Slot; P A van der Ley; R C Scheffer
Journal:  J Cell Biol       Date:  1981-06       Impact factor: 10.539

9.  Immunocytochemical localization of secretory proteins in bovine pancreatic exocrine cells.

Authors:  J P Kraehenbuhl; L Racine; J D Jamieson
Journal:  J Cell Biol       Date:  1977-02       Impact factor: 10.539

10.  Rapid release of the zymogen granule protein by osmium tetroxide and its retention during fixation by glutaraldehyde.

Authors:  A Amsterdam; M Schramm
Journal:  J Cell Biol       Date:  1966-05       Impact factor: 10.539
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  2 in total

Review 1.  On approaches to the functional restoration of salivary glands damaged by radiation therapy for head and neck cancer, with a review of related aspects of salivary gland morphology and development.

Authors:  R S Redman
Journal:  Biotech Histochem       Date:  2008-06       Impact factor: 1.718

2.  Binding sites of Ulex europaeus-lectin I in human parotid gland. A light-microscopic and ultrastructural study using the immunoperoxidase technique and immunocryoultramicrotomy.

Authors:  I A Born; K P Zimmer; K Schwechheimer; H Maier; P Möller
Journal:  Cell Tissue Res       Date:  1987-05       Impact factor: 5.249
  2 in total

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