Literature DB >> 6206084

A rapid method for the inactivation of virus infectivity prior to assay for interferons.

A D Barrett, N Hunt, N J Dimmock.   

Abstract

Virus infectivity in samples of culture medium or suspensions of animal tissue which are required for interferon assay can be rapidly and conveniently inactivated by overnight incubation with beta-propiolactone (BPL). As BPL hydrolyses spontaneously samples can be assayed with no further treatment. BPL does not affect the interfering activity of alpha, beta or gamma mouse interferons.

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Year:  1984        PMID： 6206084     DOI： 10.1016/0166-0934(84)90072-7

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  6 in total

1.  Species-independent bioassay for sensitive quantification of antiviral type I interferons.

Authors:  Thomas Kuri; Matthias Habjan; Nicola Penski; Friedemann Weber
Journal:  Virol J       Date:  2010-02-26       Impact factor: 4.099

2.  Virus Multiplicity of Infection Affects Type I Interferon Subtype Induction Profiles and Interferon-Stimulated Genes.

Authors:  Luna A Zaritsky; Jacquelyn R Bedsaul; Kathryn C Zoon
Journal:  J Virol       Date:  2015-09-09       Impact factor: 5.103

3.  The effect of interferon treatment of targets on susceptibility to cytotoxic T-lymphocyte killing: augmentation of allogeneic killing and virus-specific killing relative to viral antigen expression.

Authors:  M J Blackman; A G Morris
Journal:  Immunology       Date:  1985-11       Impact factor: 7.397

4.  Type I Interferon-Mediated Induction of Antiviral Genes and Proteins Fails to Protect Cells from the Cytopathic Effects of Sendai Virus Infection.

Authors:  Jacquelyn R Bedsaul; Luna A Zaritsky; Kathryn C Zoon
Journal:  J Interferon Cytokine Res       Date:  2016-08-10       Impact factor: 2.607

5.  Type I interferon reaction to viral infection in interferon-competent, immortalized cell lines from the African fruit bat Eidolon helvum.

Authors:  Susanne E Biesold; Daniel Ritz; Florian Gloza-Rausch; Robert Wollny; Jan Felix Drexler; Victor M Corman; Elisabeth K V Kalko; Samuel Oppong; Christian Drosten; Marcel A Müller
Journal:  PLoS One       Date:  2011-11-30       Impact factor: 3.240

6.  Fast type I interferon response protects astrocytes from flavivirus infection and virus-induced cytopathic effects.

Authors:  Richard Lindqvist; Filip Mundt; Jonathan D Gilthorpe; Silke Wölfel; Nelson O Gekara; Andrea Kröger; Anna K Överby
Journal:  J Neuroinflammation       Date:  2016-10-24       Impact factor: 8.322
  6 in total

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