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Literature DB >> 6205832

Identification of the microtubule-associated protein map2 and its binding sites on metaphase chromosomes from cultured cells.

F Wandosell, A Rodríguez-Campos, J Fernández Piqueras, J Avila.

Abstract

The microtubule-associated protein MAP2, which binds preferentially to AT sequences of DNA, can bind to metaphase chromosomes. The binding pattern of MAP2 to chromosomes is similar to that found for the binding of the bisbenzimidazole derivative 33258 Hoechst, which also binds preferentially to AT-rich regions.

Entities: Chemical Gene

Mesh：

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Year: 1984 PMID： 6205832 DOI： 10.1007/bf00352280

Source DB: PubMed Journal: Chromosoma ISSN： 0009-5915 Impact factor: 4.316

21 in total

Review 1. Ultrastructure of the mitotic spindle.

Authors: H Fuge
Journal: Int Rev Cytol Suppl Date: 1977

2. Microtubule assembly in the absence of added nucleotides.

Authors: M L Shelanski; F Gaskin; C R Cantor
Journal: Proc Natl Acad Sci U S A Date: 1973-03 Impact factor: 11.205

3. Quantitation and characterization of the microtubule associated MAP2 in porcine tissues and its isolation from porcine (PK15) and human (HeLa) cell lines.

Authors: M M Valdivia; J Avila; J Coll; C Colaço; I V Sandoval
Journal: Biochem Biophys Res Commun Date: 1982-04-29 Impact factor: 3.575

4. Binding of microtubule protein to DNA and chromatin: possibility of simultaneous linkage of microtubule to nucleic and assembly of the microtubule structure.

Authors: A Villasante; V G Corces; R Manso-Martínez; J Avila
Journal: Nucleic Acids Res Date: 1981-02-25 Impact factor: 16.971

Identification of the microtubule-associated protein map2 and its binding sites on metaphase chromosomes from cultured cells.

Review 1. Ultrastructure of the mitotic spindle.

2. Microtubule assembly in the absence of added nucleotides.

3. Quantitation and characterization of the microtubule associated MAP2 in porcine tissues and its isolation from porcine (PK15) and human (HeLa) cell lines.

4. Binding of microtubule protein to DNA and chromatin: possibility of simultaneous linkage of microtubule to nucleic and assembly of the microtubule structure.

5. Origin of kinetochore microtubules in Chinese hamster ovary cells.

6. Comparative measurement by radioimmunoassay of the brain microtubule-associated protein MAP2.

7. Microtubule-associated proteins: a monoclonal antibody to MAP2 binds to differentiated neurons.

8. Use of an 125I-labelled DNA ligand to probe DNA structure.

9. High molecular weight MAPs are part of the mitotic spindle.

10. Bridges between microtubules.

Review 1. High-Mr microtubule-associated proteins: properties and functions.

2. Relative position of constitutive heterochromatin and of nucleolar structures during mouse spermiogenesis.