Investigations on the carcinogenicity of the artificial sweeteners sodium cyclamate and sodium saccharin in rats in a two-generation experiment.

In a two-generation experiment oral lifelong administration of a 2% and a 5% mixture of sodium cyclamate and sodium saccharin in the feed did not result in carcinogenic effects in Sprague-Dawley rats (70-78 rats/group). In particular, no tumors of the urinary tract were detected, apart from one female rat with a papilloma of the urinary bladder (2% group). A sugar-receiving group, as well as an untreated control group did not yield any unexpected results. Stone formation in the urinary bladder was considerably increased in the group receiving sodium cyclamate and saccharin at 5% concentration.