Literature DB >> 6205018

Isolation of immunogenic neuraminidases of human influenza viruses by a combination of genetic and biochemical procedures.

M Gallagher, D J Bucher, R Dourmashkin, J F Davis, G Rosenn, E D Kilbourne.   

Abstract

Neuraminidases were purified from reassortant viruses (H6N1 and H6N2) containing the two antigenic subtypes (N1 and N2) found in human influenza viruses. Surface glycoproteins were solubilized with octylglucoside, and the neuraminidase was isolated by chromatography on DEAE-Sephadex. Neuraminidase isolated by this technique coeluted with viral lipids and spontaneously formed liposomes on dialysis. The purified neuraminidase was immunogenic in rabbits, producing a significant antibody response at dose levels as low as 1 microgram.

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Year:  1984        PMID： 6205018      PMCID： PMC271253          DOI： 10.1128/jcm.20.1.89-93.1984

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

1.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

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3.  Induction of partial immunity to influenza by a neuraminidase-specific vaccine.

Authors:  R B Couch; J A Kasel; J L Gerin; J L Schulman; E D Kilbourne
Journal:  J Infect Dis       Date:  1974-04       Impact factor: 5.226

4.  Influenzavirus neuraminidase and neuraminidase-inhibition test procedures.

Authors:  M Aymard-Henry; M T Coleman; W R Dowdle; W G Laver; G C Schild; R G Webster
Journal:  Bull World Health Organ       Date:  1973       Impact factor: 9.408

5.  Reactions of antibodies with surface antigens of influenza virus.

Authors:  R G Webster; W G Laver; E D Kilbourne
Journal:  J Gen Virol       Date:  1968-12       Impact factor: 3.891

6.  Association of the envelope glycoproteins of influenza virus with liposomes--a model study on viral envelope assembly.

Authors:  R T Huang; K Wahn; H D Klenk; R Rott
Journal:  Virology       Date:  1979-08       Impact factor: 3.616

7.  Identification in a recombinant influenza virus of structural proteins derived from both parents.

Authors:  W G Laver; E D Kilbourne
Journal:  Virology       Date:  1966-11       Impact factor: 3.616

8.  Reconstitution of Semliki forest virus membrane.

Authors:  A Helenius; E Fries; J Kartenbeck
Journal:  J Cell Biol       Date:  1977-12       Impact factor: 10.539

9.  Comparative efficacy of neuraminidase-specific and conventional influenza virus vaccines in induction of antibody to neuraminidase in humans.

Authors:  E D Kilbourne
Journal:  J Infect Dis       Date:  1976-10       Impact factor: 5.226

10.  Clinical and immunologic evaluation of neuraminidase-specific influenza A virus vaccine in humans.

Authors:  P L Ogra; T Chow; K R Beutner; E Rubi; J Strussenberg; S DeMello; C Rizzone
Journal:  J Infect Dis       Date:  1977-04       Impact factor: 5.226
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2.  Purified influenza virus hemagglutinin and neuraminidase are equivalent in stimulation of antibody response but induce contrasting types of immunity to infection.

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3.  Effect of priming with H1N1 influenza viruses of variable antigenic distances on challenge with 2009 pandemic H1N1 virus.

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4.  M protein (M1) of influenza virus: antigenic analysis and intracellular localization with monoclonal antibodies.

Authors:  D Bucher; S Popple; M Baer; A Mikhail; Y F Gong; C Whitaker; E Paoletti; A Judd
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5.  Oral immunization of rats with proteinoid microspheres encapsulating influenza virus antigens.

Authors:  N Santiago; S Milstein; T Rivera; E Garcia; T Zaidi; H Hong; D Bucher
Journal:  Pharm Res       Date:  1993-08       Impact factor: 4.200

6.  Complementation of recombinant baculoviruses by coinfection with wild-type virus facilitates production in insect larvae of antigenic proteins of hepatitis B virus and influenza virus.

Authors:  P M Price; C F Reichelderfer; B E Johansson; E D Kilbourne; G Acs
Journal:  Proc Natl Acad Sci U S A       Date:  1989-03       Impact factor: 11.205

7.  Humans and ferrets with prior H1N1 influenza virus infections do not exhibit evidence of original antigenic sin after infection or vaccination with the 2009 pandemic H1N1 influenza virus.

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8.  Class II major histocompatibility complex-restricted T cells specific for a virion structural protein that do not recognize exogenous influenza virus. Evidence that presentation of labile T cell determinants is favored by endogenous antigen synthesis.

Authors:  L C Eisenlohr; C J Hackett
Journal:  J Exp Med       Date:  1989-03-01       Impact factor: 14.307

9.  Avian influenza A/H5N1 neuraminidase expressed in yeast with a functional head domain.

Authors:  S Yongkiettrakul; K Boonyapakron; A Jongkaewwattana; A Wanitchang; U Leartsakulpanich; P Chitnumsub; L Eurwilaichitr; Y Yuthavong
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  9 in total

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