Literature DB >> 6204926

Mouse hepatocyte synthesis and induction of the acute phase reactant: serum amyloid P-component.

P T Le, R F Mortensen.   

Abstract

A methodology for obtaining reproducible in vitro induction of the synthesis of the acute phase reactant serum amyloid P-component (SAP) by purified mouse hepatocytes was established. Optimal hepatocyte culture conditions for the induction and synthesis of SAP required certain hormones, a substratum for cell attachment, and activated macrophages. Leibowitz L15 medium had to be supplemented with dexamethasone, indomethacin, insulin, glucose, and fetal bovine serum. Purified mouse IL 1 could substitute for activated macrophages in the induction of SAP. Hepatocytes were allowed to adhere to a collagen matrix to enhance both cell viability and SAP synthesis induced by IL 1. Elicited macrophages cultured with hepatocytes were capable of augmenting SAP synthesis in the presence of IL 1.

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Year:  1984        PMID： 6204926     DOI： 10.1007/bf02619624

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  30 in total

1.  Characterization of C-reactive protein and the complement subcomponent C1t as homologous proteins displaying cyclic pentameric symmetry (pentraxins).

Authors:  A P Osmand; B Friedenson; H Gewurz; R H Painter; T Hofmann; E Shelton
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

2.  Studies on the purification and structure-functional relationships of murine lymphocyte activating factor (Interleukin 1).

Authors:  S B Mizel
Journal:  Mol Immunol       Date:  1980-05       Impact factor: 4.407

3.  Effects of polyribonoinosinic acid polyribocytidylic acid and a mouse interferon preparation on cytochrome P-450-dependent monooxygenase systems in cultures of primary mouse hepatocytes.

Authors:  K W Renton; L B Deloria; G J Mannering
Journal:  Mol Pharmacol       Date:  1978-07       Impact factor: 4.436

4.  Biochemical studies on liver functions in primary cultured hepatocytes of adult rats. I. Hormonal effects on cell viability and protein synthesis.

Authors:  K Tanaka; M Sato; Y Tomita; A Ichihara
Journal:  J Biochem       Date:  1978-10       Impact factor: 3.387

5.  Mouse liver cell culture. II. Primary culture.

Authors:  J E Klaunig; P J Goldblatt; D E Hinton; M M Lipsky; B F Trump
Journal:  In Vitro       Date:  1981-10

6.  Murine amyloid protein AA in casein-induced experimental amyloidosis.

Authors:  M Skinner; T Shirahama; M D Benson; A S Cohen
Journal:  Lab Invest       Date:  1977-04       Impact factor: 5.662

7.  Amyloid P-component in mice injected with casein: identification in amyloid deposits and in the cytoplasm of hepatocytes.

Authors:  M L Baltz; R F Dyck; M B Pepys
Journal:  Immunology       Date:  1980-09       Impact factor: 7.397

8.  Haptoglobin and albumin synthesis in isolated rat hepatocytes. Response to potential mediators of the acute-phase reaction.

Authors:  D C Hooper; C J Steer; C A Dinarello; A C Peacock
Journal:  Biochim Biophys Acta       Date:  1981-03-26

9.  Mouse liver cell culture. I. Hepatocyte isolation.

Authors:  J E Klaunig; P J Goldblatt; D E Hinton; M M Lipsky; J Chacko; B F Trump
Journal:  In Vitro       Date:  1981-10

10.  Control of the acute phase response. Demonstration of C-reactive protein synthesis and secretion by hepatocytes during acute inflammation in the rabbit.

Authors:  I Kushner; G Feldmann
Journal:  J Exp Med       Date:  1978-08-01       Impact factor: 14.307
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  2 in total

1.  Biosynthesis of the third component of complement in rat liver epithelial cell lines and its stimulation by effector molecules from cultured human mononuclear cells.

Authors:  M Guiguet; M F Exilie Frigere; M C Dethieux; Y Bidan; G Mack
Journal:  In Vitro Cell Dev Biol       Date:  1987-12

2.  Serum amyloid P component and autoimmune parameters in the assessment of arthritis activity in MRL/lpr/lpr mice.

Authors:  C Rordorf-Adam; D Serban; A Pataki; M Grüninger
Journal:  Clin Exp Immunol       Date:  1985-09       Impact factor: 4.330
  2 in total

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