Literature DB >> 6204645

Effects of verapamil on lipolysis due to dibutyryl cyclic AMP.

H Goko, S Takashima, S Shimizu, S Kagawa, A Matsuoka.   

Abstract

The effects of verapamil, a calcium antagonist, on lipolysis in isolated rat adipocytes were studied. Verapamil (100 microM) potentiated lipolysis due to dibutyryl cyclic AMP (Bt2cAMP) at submaximal concentrations, with or without extracellular Ca2+. Lipolysis due to 0.5 mM-Bt2cAMP was potentiated by verapamil in a dose-dependent manner up to 200 microM, whereas at concentrations higher than 100 microM the stimulatory effect of verapamil was progressively diminished with or without extracellular Ca2+. Verapamil showed only an inhibitory effect on lipolysis due to adrenaline (0.1-10 microM) or 3-isobutyl-1-methylxanthine (IBMX; 25-200 microM). The stimulatory effect of verapamil on lipolysis due to Bt2cAMP was not blocked by alpha-adrenergic antagonists. These results suggest (i) that verapamil has a biphasic effect on lipolysis due to Bt2cAMP and only an inhibitory effect on that due to adrenaline or IBMX, and (ii) that extracellular Ca2+ or alpha-adrenergic receptors are not involved in the action of verapamil.

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Year:  1984        PMID： 6204645      PMCID： PMC1153626          DOI： 10.1042/bj2200321

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  25 in total

Review 1.  Regulation of lipolysis in adipose tissue.

Authors:  H Meisner; J R Carter
Journal:  Horiz Biochem Biophys       Date:  1977

Review 2.  Specific pharmacology of calcium in myocardium, cardiac pacemakers, and vascular smooth muscle.

Authors:  A Fleckenstein
Journal:  Annu Rev Pharmacol Toxicol       Date:  1977       Impact factor: 13.820

3.  The influence of extracellular calcium ion on hormone-activated lipolysis.

Authors:  R J Schimmel
Journal:  Biochim Biophys Acta       Date:  1973-11-29

4.  Evidence for a role of adenosine 3',5'-monophosphate in adipose tissue lipolysis.

Authors:  B Weiss; J I Davies; B B Brodie
Journal:  Biochem Pharmacol       Date:  1966-10       Impact factor: 5.858

5.  Biochemical evidence for pharmacological similarities between alpha-adrenoreceptors and voltage-dependent Na+ and Ca++ channels.

Authors:  C Frelin; P Vigne; M Lazdunski
Journal:  Biochem Biophys Res Commun       Date:  1982-06-15       Impact factor: 3.575

6.  Calcium and cAMP in stimulus-response coupling.

Authors:  H Rasmussen
Journal:  Ann N Y Acad Sci       Date:  1980       Impact factor: 5.691

7.  alpha-Adrenergic blockade and inhibition of A23187 mediated Ca2+ uptake by the calcium antagonist verapamil in rat liver cells.

Authors:  P F Blackmore; M F El-Refai; J H Exton
Journal:  Mol Pharmacol       Date:  1979-05       Impact factor: 4.436

8.  Interactions of D600 (methoxyverapamil) and local anesthetics with rat brain alpha-adrenergic and muscarinic receptors.

Authors:  A S Fairhurst; M L Whittaker; F J Ehlert
Journal:  Biochem Pharmacol       Date:  1980-02       Impact factor: 5.858

9.  The action of local anaesthetics on lipolysis and on adenosine 3':5'-cyclic monophosphate content in isolated rat fat-cells.

Authors:  K Siddle; C N Hales
Journal:  Biochem J       Date:  1974-08       Impact factor: 3.857

10.  Adenosine 3',5'-monophosphate in biological materials. II. The measurement of adenosine 3',5'-monophosphate in tissues and the role of the cyclic nucleotide in the lipolytic response of fat to epinephrine.

Authors:  R W Butcher; R J Ho; H C Meng; E W Sutherland
Journal:  J Biol Chem       Date:  1965-11       Impact factor: 5.157
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