Literature DB >> 620463

A simplified procedure for the analysis of cholesterol, phospholipids and bile salts in human bile.

C H Bolton, T S Low-Beer, E W Pomare, A C Wicks, J Yeates, K W Heaton.   

Abstract

Cholesterol, total bile salts and phospholipids have been measured on alcoholic extracts from human bile-rich duodenal contents. Folch extraction before phospholipid assay was found to be unnecessary and, unless fresh samples are used, it is liable to give misleadingly low values. Removal of bile pigment before measuring cholesterol was unnecessary because this estimation was done by gas-liquid chromatography. The methods described provide a simple, reproducible analysis of bile lipids.

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Year:  1978        PMID: 620463     DOI: 10.1016/0009-8981(78)90222-x

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN: 0009-8981            Impact factor:   3.786


  8 in total

1.  Effects of a new, concentrated wheat fibre preparation on intestinal transit, deoxycholic acid metabolism and the composition of bile.

Authors:  S N Marcus; K W Heaton
Journal:  Gut       Date:  1986-08       Impact factor: 23.059

2.  Effects of the fibre components pectin, cellulose, and lignin on bile salt metabolism and biliary lipid composition in man.

Authors:  L C Hillman; S G Peters; C A Fisher; E W Pomare
Journal:  Gut       Date:  1986-01       Impact factor: 23.059

3.  Diet and gall stones: effects of refined and unrefined carbohydrate diets on bile cholesterol saturation and bile acid metabolism.

Authors:  J R Thornton; P M Emmett; K W Heaton
Journal:  Gut       Date:  1983-01       Impact factor: 23.059

4.  Effects of dietary sucrose on factors influencing cholesterol gall stone formation.

Authors:  D Werner; P M Emmett; K W Heaton
Journal:  Gut       Date:  1984-03       Impact factor: 23.059

5.  A relation between high-density-lipoprotein cholesterol and bile cholesterol saturation.

Authors:  J R Thornton; K W Heaton; D G Macfarlane
Journal:  Br Med J (Clin Res Ed)       Date:  1981-11-21

6.  Do colonic bacteria contribute to cholesterol gall-stone formation? Effects of lactulose on bile.

Authors:  J R Thornton; K W Heaton
Journal:  Br Med J (Clin Res Ed)       Date:  1981-03-28

7.  Lipid composition of bile in diabetics and obesity-matched controls.

Authors:  G B Haber; K W Heaton
Journal:  Gut       Date:  1979-06       Impact factor: 23.059

8.  Intestinal transit, deoxycholic acid and the cholesterol saturation of bile--three inter-related factors.

Authors:  S N Marcus; K W Heaton
Journal:  Gut       Date:  1986-05       Impact factor: 23.059

  8 in total

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