Literature DB >> 6204553

A rapid method of concentrating proteins in small volumes with high recovery using Sephadex G-25.

A Saul, M Don.   

Abstract

A method for concentrating small (10 to 500 microliter) volumes of protein solutions by mixing in a microcentrifuge tube with measured volumes of dry Sephadex G-25 is described. The tube is centrifuged, and the protein solution concentrated up to fivefold passes through a small hole in the base of the tube and is collected. Recovery of protein is 90-95%.

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Year:  1984        PMID: 6204553     DOI: 10.1016/0003-2697(84)90838-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  4 in total

1.  Catalytic activity of maize leaf phosphoenolpyruvate carboxylase in relation to oligomerization.

Authors:  G H Walker; M S Ku; G E Edwards
Journal:  Plant Physiol       Date:  1986-04       Impact factor: 8.340

2.  Purification and Characterization of Three Chitosanase Activities from Bacillus megaterium P1.

Authors:  A Pelletier; J Sygusch
Journal:  Appl Environ Microbiol       Date:  1990-04       Impact factor: 4.792

3.  Topographical analysis of the G virion of Aleutian mink disease parvovirus with monoclonal antibodies.

Authors:  D L Barnard; F B Johnson
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

4.  Myosin II phosphorylation and the dynamics of stress fibers in serum-deprived and stimulated fibroblasts.

Authors:  K A Giuliano; J Kolega; R L DeBiasio; D L Taylor
Journal:  Mol Biol Cell       Date:  1992-09       Impact factor: 4.138

  4 in total

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