Literature DB >> 6204381

Sindbis virus mutants selected for rapid growth in cell culture display attenuated virulence in animals.

R A Olmsted, R S Baric, B A Sawyer, R E Johnston.   

Abstract

Mutants of Sindbis virus were selected for rapid growth in baby hamster kidney (BHK) cell cultures and screened for attenuation of virulence in suckling mice. Comparisons among independently isolated virulent and attenuated strains, as well as a classical reversion analysis, showed that accelerated penetration of BHK cells was correlated with attenuation in vivo. Both phenotypic changes resulted from a reorganization of virion structure as detected by monoclonal antibodies. These results suggest that mutants selected for rapid growth in cell culture may be useful as attenuated vaccines and for studies of the molecular basis of virus pathogenesis.

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Year:  1984        PMID： 6204381     DOI： 10.1126/science.6204381

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  32 in total

1.  Expression of the F and HN glycoproteins of human parainfluenza virus type 3 by recombinant vaccinia viruses: contributions of the individual proteins to host immunity.

Authors:  M K Spriggs; B R Murphy; G A Prince; R A Olmsted; P L Collins
Journal:  J Virol       Date:  1987-11       Impact factor: 5.103

2.  Mutational analysis of a virulence locus in the E2 glycoprotein gene of Sindbis virus.

Authors:  J M Polo; R E Johnston
Journal:  J Virol       Date:  1991-11       Impact factor: 5.103

3.  Antibody-selected variation and reversion in Sindbis virus neutralization epitopes.

Authors:  D S Stec; A Waddell; C S Schmaljohn; G A Cole; A L Schmaljohn
Journal:  J Virol       Date:  1986-03       Impact factor: 5.103

4.  Attenuating mutations in glycoproteins E1 and E2 of Sindbis virus produce a highly attenuated strain when combined in vitro.

Authors:  J M Polo; R E Johnston
Journal:  J Virol       Date:  1990-09       Impact factor: 5.103

5.  Cost of host radiation in an RNA virus.

Authors:  P E Turner; S F Elena
Journal:  Genetics       Date:  2000-12       Impact factor: 4.562

Review 6.  The alphaviruses: gene expression, replication, and evolution.

Authors:  J H Strauss; E G Strauss
Journal:  Microbiol Rev       Date:  1994-09

7.  Sindbis virus attachment: isolation and characterization of mutants with impaired binding to vertebrate cells.

Authors:  J Dubuisson; C M Rice
Journal:  J Virol       Date:  1993-06       Impact factor: 5.103

8.  Differential induction of type I interferon responses in myeloid dendritic cells by mosquito and mammalian-cell-derived alphaviruses.

Authors:  Reed S Shabman; Thomas E Morrison; Christopher Moore; Laura White; Mehul S Suthar; Linda Hueston; Nestor Rulli; Brett Lidbury; Jenny P-Y Ting; Suresh Mahalingam; Mark T Heise
Journal:  J Virol       Date:  2006-11-01       Impact factor: 5.103

9.  Structural rearrangement of infecting Sindbis virions at the cell surface: mapping of newly accessible epitopes.

Authors:  W J Meyer; R E Johnston
Journal:  J Virol       Date:  1993-09       Impact factor: 5.103

10.  Expression of the F glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the F and G glycoproteins to host immunity.

Authors:  R A Olmsted; N Elango; G A Prince; B R Murphy; P R Johnson; B Moss; R M Chanock; P L Collins
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205
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