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Literature DB >> 6201493

A domain-specific marker for the hepatocyte plasma membrane. II. Ultrastructural localization of leucine aminopeptidase to the bile canalicular domain of isolated rat liver plasma membranes.

Abstract

Leucine aminopeptidase (LAP) is an integral membrane glycoprotein localized to the apical membrane domain of intestinal and kidney epithelial cells. By indirect immunofluorescence, we have shown that antibodies raised against rat intestinal LAP recognized a similar protein concentrated in the bile canalicular (BC) domain of the hepatocyte in situ (Roman, L.M., and A.L. Hubbard, 1983, J. Cell Biol., 96:1548-1558). We have extended this localization to the ultrastructural level. When a saponin-permeabilized, agarose-embedded plasma membrane (PM) fraction was incubated with affinity-purified anti-LAP, 85% of the protein A-gold particles associated with the three recognizable PM domains were present in the BC. The levels of labeling on the other two domains (sinusoidal and lateral) did not exceed that observed with nonimmune controls. The concentration of LAP in the BC domain in isolated PM sheets prompted us to use this antigen for the affinity isolation of BC membrane (Roman, L.M., and A.L. Hubbard, 1984, J. Cell Biol., 98:1497-1504, companion paper).

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Year: 1984 PMID： 6201493 PMCID： PMC2113232 DOI： 10.1083/jcb.98.4.1488

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

21 in total

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Journal: Biochim Biophys Acta Date: 1973-02-16

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Journal: J Cell Biol Date: 1967-01 Impact factor: 10.539

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Journal: J Cell Biol Date: 1969-07 Impact factor: 10.539

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Journal: J Cell Biol Date: 1979-10 Impact factor: 10.539

12 in total

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Journal: Biochem J Date: 1990-10-01 Impact factor: 3.857

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Journal: Physiol Genomics Date: 2011-03-22 Impact factor: 3.107

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Journal: Gastroenterol Jpn Date: 1986-06

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Authors: K S Matlin; K Simons
Journal: J Cell Biol Date: 1984-12 Impact factor: 10.539

10. A domain-specific marker for the hepatocyte plasma membrane. III. Isolation of bile canalicular membrane by immunoadsorption.

Authors: L M Roman; A L Hubbard
Journal: J Cell Biol Date: 1984-04 Impact factor: 10.539