Literature DB >> 6200963

Embedding iliac bone biopsies at low temperature using glycol and methyl methacrylates.

D Chappard, C Alexandre, M Camps, J P Montheard, G Riffat.   

Abstract

A mixture of pure and anhydrous glycol methacrylate and methyl methacrylate is used as an embedding medium for iliac bone biopsies. Infiltration is carried out at -20 C with the embedding medium and a cold inactivated catalyst-initiator system. Raising the temperature to 4 C initiates polymerization and limits the peak temperature of polymerization to 25 C. In this way, such thermolabile enzymes as osteoclastic acid phosphatase are preserved. After staining, sections are dehydrated in polyethylene glycol 400 30% in 2-propanol. This gives flat sections and improves staining properties.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6200963     DOI： 10.3109/10520298309066802

Source DB:  PubMed          Journal:  Stain Technol        ISSN： 0038-9153


  12 in total

1.  Bone tissue response to four-month antiorthostatic bedrest: a bone histomorphometric study.

Authors:  S Palle; L Vico; S Bourrin; C Alexandre
Journal:  Calcif Tissue Int       Date:  1992-09       Impact factor: 4.333

2.  Bone mass and bone cellular variations after five months of physical training in rhesus monkeys: histomorphometric study.

Authors:  S Bourrin; E Zerath; L Vico; C Milhaud; C Alexandre
Journal:  Calcif Tissue Int       Date:  1992-05       Impact factor: 4.333

3.  Recruitment of new osteoblasts and osteoclasts is the earliest critical event in the pathogenesis of human multiple myeloma.

Authors:  R Bataille; D Chappard; C Marcelli; P Dessauw; P Baldet; J Sany; C Alexandre
Journal:  J Clin Invest       Date:  1991-07       Impact factor: 14.808

4.  Ineffectiveness of calcitonin on a local-disuse osteoporosis in the sheep: a histomorphometric study.

Authors:  T Thomas; T M Skerry; L Vico; F Caulin; L E Lanyon; C Alexandre
Journal:  Calcif Tissue Int       Date:  1995-09       Impact factor: 4.333

5.  Trabecular and endocortical bone remodeling in postmenopausal osteoporosis: comparison with normal postmenopausal women.

Authors:  M E Arlot; P D Delmas; D Chappard; P J Meunier
Journal:  Osteoporos Int       Date:  1990-10       Impact factor: 4.507

6.  A methacrylate embedding technique for combined autoradiography and acid phosphatase histochemistry.

Authors:  G H Lewis; I D Bowen
Journal:  Histochem J       Date:  1985-04

7.  A new histological embedding method by low-temperature polymerisation of methyl methacrylate allowing immuno- and enzyme histochemical studies on semi-thin sections of undecalcified bone marrow biopsies.

Authors:  J Bernhards; B Weitzel; M Werner; M Rimpler; A Georgii
Journal:  Histochemistry       Date:  1992-10

8.  Effects of a one-year administration of phosphate and intermittent calcitonin on bone-forming and bone-resorbing cells in involutional osteoporosis: a histomorphometric study.

Authors:  C Alexandre; D Chappard; F Caulin; A Bertrand; S Palle; G Riffat
Journal:  Calcif Tissue Int       Date:  1988-06       Impact factor: 4.333

9.  Hyperparathyroidism in proximal femur fractures biological and histomorphometric study in 21 patients over 75 years old.

Authors:  C L Benhamou; D Chappard; J B Gauvain; M Popelier; C Roux; G Picaper; C Alexandre
Journal:  Clin Rheumatol       Date:  1991-06       Impact factor: 2.980

10.  Hypocalcemia response following calcitonin administration: lack of correlation with osteoclast number determined after histoenzymologic identification in osteoporosis.

Authors:  S Palle; D Chappard; L Vico; C Alexandre
Journal:  Clin Rheumatol       Date:  1988-09       Impact factor: 2.980
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.