Literature DB >> 620023

Coupling polylysine to glass beads for plasma membrane isolation.

B S Jacobson, J Cronin, D Branton.   

Abstract

Solid glass beads for use in isolating cell membranes were coated with a stable, covalently attached layer of polylysine. The optimal conditions for coating the bead surface were established and the beads were tested by measuring the attachment of human erythrocyte plasma membranes. When compared to other beads, such as those with absorbed polylysine or protamine, none retained red-cell membranes as well as glass beads with covalently linked polylysine.

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Year:  1978        PMID: 620023     DOI: 10.1016/0005-2736(78)90436-4

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN: 0006-3002


  14 in total

1.  Dynamics of nonspecific adsorption of insulin to erythrocyte membranes.

Authors:  R M Fulbright; D Axelrod
Journal:  J Fluoresc       Date:  1993-03       Impact factor: 2.217

2.  An adhesion-based method for plasma membrane isolation: evaluating cholesterol extraction from cells and their membranes.

Authors:  Ludmila Bezrukov; Paul S Blank; Ivan V Polozov; Joshua Zimmerberg
Journal:  Anal Biochem       Date:  2009-07-22       Impact factor: 3.365

3.  Subnanosecond polarized fluorescence photobleaching: rotational diffusion of acetylcholine receptors on developing muscle cells.

Authors:  Y Yuan; D Axelrod
Journal:  Biophys J       Date:  1995-08       Impact factor: 4.033

4.  Quantitation of Na+/K(+)-ATPase and glucose transporter isoforms in rat adipocyte plasma membrane by immunogold labeling.

Authors:  M Voldstedlund; J Tranum-Jensen; J Vinten
Journal:  J Membr Biol       Date:  1993-10       Impact factor: 1.843

5.  Cell poking. Determination of the elastic area compressibility modulus of the erythrocyte membrane.

Authors:  B Daily; E L Elson; G I Zahalak
Journal:  Biophys J       Date:  1984-04       Impact factor: 4.033

6.  Immobilization of a lactase onto a magnetic support by covalent attachment to polyethyleneimine-glutaraldehyde-activated magnetite.

Authors:  R F Dekker
Journal:  Appl Biochem Biotechnol       Date:  1989-12       Impact factor: 2.926

7.  Structural investigation of biological material in aqueous environment by means of infrared-ATR spectroscopy.

Authors:  P Hofer; U P Fringeli
Journal:  Biophys Struct Mech       Date:  1979-12

8.  Immobilization of rat brain synaptic vesicles on positively-charged glass microspheres.

Authors:  J K Disbrow; M J Gershten; J A Ruth
Journal:  Experientia       Date:  1983-06-15

9.  Reversible binding kinetics of a cytoskeletal protein at the erythrocyte submembrane.

Authors:  A L Stout; D Axelrod
Journal:  Biophys J       Date:  1994-09       Impact factor: 4.033

10.  Growth of anchorage dependent mammalian cells on glycine-derivatized polystyrene in suspension culture.

Authors:  M J Kuo; C Lewis; R A Martin; R E Miller; R A Schoenfeld; J M Schuck; B S Wildi
Journal:  In Vitro       Date:  1981-10
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