Literature DB >> 6199425

Immunoaffinity purification of human complement component C9 using monoclonal antibodies.

B P Morgan, R A Daw, K Siddle, J P Luzio, A K Campbell.   

Abstract

Five monoclonal antibodies to human C9 were produced. Three of the antibodies had affinities from 1.6 to 3.1 X 10(9) M-1 and 2 were of lower affinity, 0.1 to 0.3 X 10(9) M-1. Four distinct antigenic sites on C9 were detected. The lowest affinity antibody was immobilised on Sepharose 4B and used to isolate C9 from human plasma. A 1200-fold purification was obtained with yield of 30-40%. The purified antigen maintained full cytolytic activity.

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Year:  1983        PMID： 6199425     DOI： 10.1016/0022-1759(83)90434-9

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  32 in total

1.  Streptococcal inhibitor of complement (SIC) inhibits the membrane attack complex by preventing uptake of C567 onto cell membranes.

Authors:  B A Fernie-King; D J Seilly; C Willers; R Würzner; A Davies; P J Lachmann
Journal:  Immunology       Date:  2001-07       Impact factor: 7.397

2.  An abnormal but functionally active complement component C9 protein found in an Irish family with subtotal C9 deficiency.

Authors:  Ann Orren; Ann M O'Hara; B Paul Morgan; Anthony P Moran; Reinhard Würzner
Journal:  Immunology       Date:  2003-03       Impact factor: 7.397

3.  Characterization of the membrane attack complex inhibitory protein CD59 antigen on human amniotic cells and in amniotic fluid.

Authors:  I A Rooney; B P Morgan
Journal:  Immunology       Date:  1992-08       Impact factor: 7.397

4.  Human rheumatoid synovial cell stimulation by the membrane attack complex and other pore-forming toxins in vitro: the role of calcium in cell activation.

Authors:  R H Daniels; B D Williams; B P Morgan
Journal:  Immunology       Date:  1990-11       Impact factor: 7.397

5.  Binding of human and rat CD59 to the terminal complement complexes.

Authors:  T Lehto; B P Morgan; S Meri
Journal:  Immunology       Date:  1997-01       Impact factor: 7.397

6.  Non-lethal complement-membrane attack on human neutrophils: transient cell swelling and metabolic depletion.

Authors:  B P Morgan
Journal:  Immunology       Date:  1988-01       Impact factor: 7.397

7.  Antigenic crossreactivity of the alpha subunit of complement component C8 with the cysteine-rich domain shared by complement component C9 and low density lipoprotein receptor.

Authors:  J Tschopp; T E Mollnes
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

8.  The preparation and characterization of monoclonal antibodies to human complement component C8 and their use in purification of C8 and C8 subunits.

Authors:  A Abraha; B P Morgan; J P Luzio
Journal:  Biochem J       Date:  1988-04-01       Impact factor: 3.857

9.  Inhibition of the formation of the complement membrane-attack complex by a monoclonal antibody to the complement component C8 alpha subunit.

Authors:  A Abraha; J P Luzio
Journal:  Biochem J       Date:  1989-12-15       Impact factor: 3.857

10.  Ectocytosis caused by sublytic autologous complement attack on human neutrophils. The sorting of endogenous plasma-membrane proteins and lipids into shed vesicles.

Authors:  J M Stein; J P Luzio
Journal:  Biochem J       Date:  1991-03-01       Impact factor: 3.857
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