Literature DB >> 6198129

A rapid, vital staining procedure for flow cytometric analysis of human reticulocytes.

B H Sage, J P O'Connell, T J Mercolino.   

Abstract

A fluorescent staining procedure based upon thioflavin T is described. This dye permits rapid, vital, nonprecipitative staining of human reticulocytes. Flow cytometric analysis of thioflavin T stained whole blood specimens results in reticulocyte counts that correlate well with new methylene blue reticulocyte counts and give coefficients of variation approaching the theoretical limit. The shape of the fluorescence histogram is qualitatively similar to the reticulocyte age profile. Paired new methylene blue and thioflavin T reticulocyte counts are presented with illustrative cases of reticulocyte maturity distributions.

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Year:  1983        PMID： 6198129     DOI： 10.1002/cyto.990040306

Source DB:  PubMed          Journal:  Cytometry        ISSN： 0196-4763


  5 in total

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2.  Changes in erythropoiesis due to radiation or chemotherapy as studied by flow cytometric determination of peripheral blood reticulocytes.

Authors:  H J Tanke; P H van Vianen; F M Emiliani; I Neuteboom; N de Vogel; A D Tates; E A de Bruijn; A T van Oosterom
Journal:  Histochemistry       Date:  1986

3.  Modelling of amyloid beta-peptide induced lesions using roller-drum incubation of hippocampal slice cultures from neonatal rats.

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4.  Thioflavin T displays enhanced fluorescence selectively inside anionic micelles and mammalian cells.

Authors:  Satish Kumar; Atul K Singh; G Krishnamoorthy; Rajaram Swaminathan
Journal:  J Fluoresc       Date:  2008-05-28       Impact factor: 2.217

5.  Significant biochemical, biophysical and metabolic diversity in circulating human cord blood reticulocytes.

Authors:  Benoît Malleret; Fenggao Xu; Narla Mohandas; Rossarin Suwanarusk; Cindy Chu; Juliana A Leite; Kayen Low; Claudia Turner; Kanlaya Sriprawat; Rou Zhang; Olivier Bertrand; Yves Colin; Fabio T M Costa; Choon Nam Ong; Mah Lee Ng; Chwee Teck Lim; Francois Nosten; Laurent Rénia; Bruce Russell
Journal:  PLoS One       Date:  2013-10-08       Impact factor: 3.240
  5 in total

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