| Literature DB >> 6197900 |
Abstract
Three ultrasensitive protein silver-staining methods have been compared with respect to the detection of tRNA in polyacrylamide gels. The method of Sammons (D. W. Sammons, L.D. Adams, and E.E. Nishizawa (1981) Electrophoresis 2, 135-141) has been shown to have remarkable sensitivity, with a detection limit of 0.3 ng tRNA/mm2, allowing the two-dimensional fractionation of submicrogram amounts of bulk tRNA. The application of this technique to developmental and differentiation problems and other areas where the amounts of nonradioactive tRNA available are limited is anticipated.Entities:
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Year: 1983 PMID： 6197900 DOI： 10.1016/0003-2697(83)90281-6
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365