Literature DB >> 6197393

Cuprolinic Blue: a specific dye for single-stranded RNA in the presence of magnesium chloride. II. Practical applications for light microscopy.

D Mendelson, J Tas, J James.   

Abstract

The application of the new nucleic acid dye Cuprolinic Blue to cell smears and tissue sections has been described. Without added cations, Cuprolinic Blue stains both DNA and RNA, whereas in the presence of 1 M MgCl2, Cuprolinic Blue specifically stains single-stranded RNA only. The total RNA can be stained after removal of DNA by DNAase digestion. Fixation in a modified Carnoy solution gave optimal staining results in all cases tested. By cytophotometry, a reliable and reproducible relative estimate can be obtained of the total nucleic acid content, the total RNA content and the amount of single-stranded RNA alone per cell.

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Year:  1983        PMID： 6197393     DOI： 10.1007/bf01003974

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  9 in total

1.  Ultramicrochemical determination of nucleic acids in individual cells using the Zeiss UMSP-I microspectrophotometer. Application to isolated rat hepatocytes of different ploidy classes.

Authors:  R C Roozemond
Journal:  Histochem J       Date:  1976-11

2.  The quantitative histochemistry of ribonucleic acid using gallocyanin.

Authors:  A Brown; C L Scholtz
Journal:  Stain Technol       Date:  1979-03

3.  The naphthol yellow S stain for proteins tested in a model system of polyacrylamide films and evaluated for practical use in histochemistry.

Authors:  J Tas; P Oud; J James
Journal:  Histochemistry       Date:  1974

4.  Histochemistry of Alcian blue. 3. The molecular biological basis of staining by Alcian blue 8GX and analogous phthalocyanins.

Authors:  J E Scott
Journal:  Histochemie       Date:  1972

5.  Conformation of RNA in situ as studied by acridine orange staining and automated cytofluorometry.

Authors:  Z Darzynkiewicz; F Traganos; T Sharpless; M R Melamed
Journal:  Exp Cell Res       Date:  1975-10-01       Impact factor: 3.905

6.  Internal organization of the ribosome.

Authors:  R I Cotter; P McPhie; W B Gratzer
Journal:  Nature       Date:  1967-12-02       Impact factor: 49.962

7.  Cuprolinic Blue: a specific dye for single-stranded RNA in the presence of magnesium chloride. I. Fundamental aspects.

Authors:  J Tas; D Mendelson; C J Noorden
Journal:  Histochem J       Date:  1983-08

8.  Protein staining methods in quantitative cytochemistry.

Authors:  J Tas; M van der Ploeg; J P Mitchell; N S Cohn
Journal:  J Microsc       Date:  1980-08       Impact factor: 1.758

9.  Adaptation of the Naphthol Yellow S staining for objects with high protein content.

Authors:  J Tas; J James; M van der Ploeg
Journal:  Histochemistry       Date:  1978-04-04
  9 in total
  5 in total

Review 1.  Detection of metabolic changes in hepatocytes by quantitative cytochemistry.

Authors:  J James; W M Frederiks; C J van Noorden; J Tas
Journal:  Histochemistry       Date:  1986

2.  Notable postnatal alterations in the myenteric plexus of normal human bowel.

Authors:  T Wester; D S O'Briain; P Puri
Journal:  Gut       Date:  1999-05       Impact factor: 23.059

3.  Differential staining of glycosaminoglycans in the predentine and dentine of rat incisor using cuprolinic blue at various magnesium chloride concentrations.

Authors:  M Goldberg; D S Septier
Journal:  Histochem J       Date:  1992-09

4.  Metachromatic staining and electron dense reaction of glycosaminoglycans by means of cuprolinic blue.

Authors:  A Juarranz; J M Ferrer; A Tato; M Cañete; J C Stockert
Journal:  Histochem J       Date:  1987-01

5.  Cuprolinic blue visualization of cytosolic and membrane-associated glycosaminoglycans in the rat junctional epithelium and gingival epithelia.

Authors:  C Ayanoglou; S Lécolle; D Septier; M Goldberg
Journal:  Histochem J       Date:  1994-03
  5 in total

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