Literature DB >> 6196989

Comparison of two methods of high-molecular-weight DNA isolation from human leucocytes.

C Gautreau, C Rahuel, J P Cartron, G Lucotte.   

Abstract

Among the different methods for isolation of high-molecular-weight DNA from leucocytes, two of them were retained as being the most interesting and were compared. This choice was based on three criteria: the quality of the DNA obtained (high-molecular-weight DNA, RNA, and protein free), the efficiency of the method (in terms of the yield of DNA obtained), and the ease and length of the method. The first method described was chosen as a reference (100% efficiency); in comparison, the second one had a 70% efficiency, but was shorter and easier to handle.

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Year:  1983        PMID： 6196989     DOI： 10.1016/0003-2697(83)90304-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  A DNA probe detecting multiple haplotypes of the human Y chromosome.

Authors:  K Y Ngo; G Vergnaud; C Johnsson; G Lucotte; J Weissenbach
Journal:  Am J Hum Genet       Date:  1986-04       Impact factor: 11.025

2.  DNA polymorphisms associated with HLA-B-like genes and evidence for a duplication of B40 genes detected with an HLA-B-specific DNA probe.

Authors:  M Mariotti; C Auffray; G Lucotte
Journal:  Immunology       Date:  1987-03       Impact factor: 7.397

3.  Restriction fragment length polymorphism of the human c-fms gene.

Authors:  D Q Xu; S Guilhot; F Galibert
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

4.  Restriction fragment length polymorphism caused by a deletion within the human c-abl gene (ABL).

Authors:  D Q Xu; F Galibert
Journal:  Proc Natl Acad Sci U S A       Date:  1986-05       Impact factor: 11.205

5.  A transthyretin variant (alanine 71) associated with familial amyloidotic polyneuropathy in a French family.

Authors:  M D Benson; J C Turpin; G Lucotte; S Zeldenrust; B LeChevalier; M D Benson
Journal:  J Med Genet       Date:  1993-02       Impact factor: 6.318

6.  Detection of Aspergillus fumigatus by polymerase chain reaction.

Authors:  C Spreadbury; D Holden; A Aufauvre-Brown; B Bainbridge; J Cohen
Journal:  J Clin Microbiol       Date:  1993-03       Impact factor: 5.948
  6 in total

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