Literature DB >> 6196045

The use of the dye neutral red as a specific, non-toxic, intra-vital stain of islets of Langerhans.

D W Gray, P R Millard, P McShane, P J Morris.   

Abstract

The development of improved methods of islet isolation from the pancreas is hampered by difficulty in identification of islets. A method described by Bensley in 1911 uses neutral red to stain the islets selectively. In the present study the method has been simplified and selective staining of islets in the rat, dog and pig demonstrated by use of light and electron microscopy. Staining of islets with neutral red does not appear to affect their viability as judged by insulin secretion and transplantation in rats.

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Year:  1983        PMID： 6196045      PMCID： PMC2040820     

Source DB:  PubMed          Journal:  Br J Exp Pathol        ISSN： 0007-1021


  7 in total

1.  Vital staining with neutral red and trypan blue of 3H-thymidine-labeled cells prior to autoradiography.

Authors:  W Sawicki; J Kieler; P Briand
Journal:  Stain Technol       Date:  1967-05

2.  The use of neutral red and Evans blue for live-dead determinations of marine plankton (with comments on the use of rotenone for inhibition of grazing).

Authors:  R W Crippen; J L Perrier
Journal:  Stain Technol       Date:  1974-03

3.  Staining by neutral red and trypan blue in sequence for assaying vital and nonvital cultured cells.

Authors:  F A DeRenzis; A Schechtman
Journal:  Stain Technol       Date:  1973-05

4.  Transplantation of intact pancreatic islets in rats.

Authors:  W F Ballinger; P E Lacy
Journal:  Surgery       Date:  1972-08       Impact factor: 3.982

5.  A sensitive, precise radioimmunoassay of serum insulin relying on charcoal separation of bound and free hormone moieties.

Authors:  J D Albano; R P Ekins; G Maritz; R C Turner
Journal:  Acta Endocrinol (Copenh)       Date:  1972-07

6.  An improved technique for the isolation and identification of mammalian islets of Langerhans.

Authors:  R Downing; D W Scharp; W F Ballinger
Journal:  Transplantation       Date:  1980       Impact factor: 4.939

7.  Islet transplantation in experimental diabetes of the rat. V. Regression of glomerular lesions in diabetic rats after intraportal transplantation of isogeneic islets. Preliminary results.

Authors:  K Federlin; R G Bretzel; U Schmidtchen
Journal:  Horm Metab Res       Date:  1976-09       Impact factor: 2.936
  7 in total
  5 in total

1.  A Historical Perspective on the Identification of Cell Types in Pancreatic Islets of Langerhans by Staining and Histochemical Techniques.

Authors:  Denis G Baskin
Journal:  J Histochem Cytochem       Date:  2015-08       Impact factor: 2.479

2.  [Relation of implanted beta cell mass and metabolic status following transplantation of pancreatic fragments in the dog].

Authors:  U J Hesse
Journal:  Langenbecks Arch Chir       Date:  1987

3.  Effects of neutral red assisted viability assessment on the cryotolerance of isolated bovine preantral follicles.

Authors:  A Langbeen; E P A Jorssen; N Granata; E Fransen; J L M R Leroy; P E J Bols
Journal:  J Assist Reprod Genet       Date:  2014-10-02       Impact factor: 3.412

4.  [In vitro and in vivo studies of isolation, transplantation and function of Langerhans islets in the swine].

Authors:  U J Hesse; J Danis; J Weyer; G Meyer; S Saad
Journal:  Langenbecks Arch Chir       Date:  1990

5.  Low dose bitter leaf improves sperm quality disrupted in immunosuppressed Wistar rats: An experimental study.

Authors:  Risikat Eniola Kadir; Abdulmumin Ibrahim; Balkis Abimbola Ibrahim; Sadiya Musa Gwadabe; Rukayat Jaji-Sulaimon; Munirat Foyeke Adigun; Adeoye Oyetunji Oyewopo
Journal:  Int J Reprod Biomed       Date:  2020-03-29
  5 in total

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