Literature DB >> 6193735

Detection of cellulase activity in polyacrylamide gels using Congo red-stained agar replicas.

P Béguin.   

Abstract

Bands that have cellulolytic activity are visualized after polyacrylamide gel electrophoresis by laying the slab gel on top of a thin sheet of 2% agar containing 0.1% carboxymethylcellulose. After a suitable incubation time, zones of carboxymethylcellulose hydrolysis are revealed by staining the agar replica with Congo red.

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Year:  1983        PMID： 6193735     DOI： 10.1016/0003-2697(83)90178-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  59 in total

1.  Is Pseudobutyrivibrio xylanivorans strain Mz5T suitable as a probiotic? An in vitro study.

Authors:  T Cepeljnik; M Zorec; R Kostanjsek; F V Nekrep; R Marinsek-Logar
Journal:  Folia Microbiol (Praha)       Date:  2003       Impact factor: 2.099

2.  Cloning, sequence analysis, and expression of genes encoding xylan-degrading enzymes from the thermophile "Caldocellum saccharolyticum".

Authors:  E Lüthi; D R Love; J McAnulty; C Wallace; P A Caughey; D Saul; P L Bergquist
Journal:  Appl Environ Microbiol       Date:  1990-04       Impact factor: 4.792

3.  Sequencing of a 1,3-1,4-beta-D-glucanase (lichenase) from the anaerobic fungus Orpinomyces strain PC-2: properties of the enzyme expressed in Escherichia coli and evidence that the gene has a bacterial origin.

Authors:  H Chen; X L Li; L G Ljungdahl
Journal:  J Bacteriol       Date:  1997-10       Impact factor: 3.490

4.  Regulation and Characterization of Xylanolytic Enzymes of Thermoanaerobacterium saccharolyticum B6A-RI.

Authors:  Y E Lee; S E Lowe; J G Zeikus
Journal:  Appl Environ Microbiol       Date:  1993-03       Impact factor: 4.792

5.  Molecular cloning and transcriptional and expression analysis of engO, encoding a new noncellulosomal family 9 enzyme, from Clostridium cellulovorans.

Authors:  Sung Ok Han; Hideaki Yukawa; Masayuki Inui; Roy H Doi
Journal:  J Bacteriol       Date:  2005-07       Impact factor: 3.490

6.  Xyn11A, a multidomain multicatalytic enzyme from Pseudobutyrivibrio xylanivorans Mz5T.

Authors:  T Cepeljnik; M T Rincón; H J Flint; R Marinsek-Logar
Journal:  Folia Microbiol (Praha)       Date:  2006       Impact factor: 2.099

7.  Enzyme diversity of the cellulolytic system produced by Clostridium cellulolyticum explored by two-dimensional analysis: identification of seven genes encoding new dockerin-containing proteins.

Authors:  Jean-Charles Blouzard; Caroline Bourgeois; Pascale de Philip; Odile Valette; Anne Bélaïch; Chantal Tardif; Jean-Pierre Bélaïch; Sandrine Pagès
Journal:  J Bacteriol       Date:  2007-01-05       Impact factor: 3.490

8.  Purification and characterization of an endoglucanase from Streptomyces lividans 66 and DNA sequence of the gene.

Authors:  M Théberge; P Lacaze; F Shareck; R Morosoli; D Kluepfel
Journal:  Appl Environ Microbiol       Date:  1992-03       Impact factor: 4.792

9.  Isolation and expression of the xynB gene and its product, XynB, a consistent component of the Clostridium cellulovorans cellulosome.

Authors:  Sung Ok Han; Hideaki Yukawa; Masayuki Inui; Roy H Doi
Journal:  J Bacteriol       Date:  2004-12       Impact factor: 3.490

10.  Recognition specificity of the duplicated segments present in Clostridium thermocellum endoglucanase CelD and in the cellulosome-integrating protein CipA.

Authors:  S Salamitou; O Raynaud; M Lemaire; M Coughlan; P Béguin; J P Aubert
Journal:  J Bacteriol       Date:  1994-05       Impact factor: 3.490
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