Literature DB >> 6193726

A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution.

F G Nobrega, C L Dieckmann, A Tzagoloff.   

Abstract

A method is described for detecting specific transcripts in crude mixtures of RNA. The method employs hybridization of single-stranded or double-stranded radioactive DNA probes in solution, followed by electrophoretic separation of the hybrid and probe on agarose and visualization by radioautography. The procedure offers the advantages of decreased preparation time and increased sensitivity over currently used methods.

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Year:  1983        PMID： 6193726     DOI： 10.1016/0003-2697(83)90145-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  4 in total

1.  Chimeric mouse-human IgG1 antibody that can mediate lysis of cancer cells.

Authors:  A Y Liu; R R Robinson; K E Hellström; E D Murray; C P Chang; I Hellström
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

2.  Genes for the alpha and beta subunits of phycocyanin.

Authors:  R de Lorimier; D A Bryant; R D Porter; W Y Liu; E Jay; S E Stevens
Journal:  Proc Natl Acad Sci U S A       Date:  1984-12       Impact factor: 11.205

3.  Immunological assays for chemokine detection in in-vitro culture of CNS cells.

Authors:  Supriya D. Mahajan; Stanley A. Schwartz; Madhavan P.N. Nair
Journal:  Biol Proced Online       Date:  2003-04-07       Impact factor: 3.244

4.  In vitro toxicity and efficacy of verdinexor, an exportin 1 inhibitor, on opportunistic viruses affecting immunocompromised individuals.

Authors:  Douglas G Widman; Savanna Gornisiewicz; Sharon Shacham; Sharon Tamir
Journal:  PLoS One       Date:  2018-10-17       Impact factor: 3.240
  4 in total

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