Site of the ganglion blocking action of gardneramine and hirsutine in the dog urinary bladder in situ preparation.

The ganglion blocking site of gardneramine (GA) and hirsutine (HS) was studied in the dog urinary bladder in an in situ preparation. GA and HS selectively inhibited the DMPP-induced contraction without having an antagonistic effect on the McN-A-343-induced and acetylcholine-induced contraction. In addition, since GA and HS showed a local anesthetic action weaker than that of procaine, the effect of procaine was studied on the same preparation. Procaine inhibited the McN-A-343-induced contraction, and it slightly inhibited the DMPP-induced and acetylcholine-induced contraction. From these findings, it is concluded that GA and HS inhibited the ganglionic transmission of the dog urinary bladder and that the blockade of the nicotinic receptor played a main role.