Literature DB >> 6193197

Hoechst 33258: a fluorescent nuclear counterstain suitable for double-labelling immunofluorescence.

D R Bainbridge, M M Macey.   

Abstract

Counterstaining with the chromosomal dye Hoechst 33258 is a simple procedure that provides an excellent general purpose nuclear counterstain for immunofluorescent work. Benefits from its use include the ready identification and orientation of structures, differentiation of lymphoid and non-lymphoid cells and easy assessment of frequency in specifically labelled cellular subpopulations. It can be used at the same time as FITC and TRITC double-labelling immunofluorescence.

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Year:  1983        PMID: 6193197     DOI: 10.1016/0022-1759(83)90246-6

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  2 in total

1.  Antibodies to cardiolipin and intermediate filaments: a study of autoimmunity in rheumatoid arthritis.

Authors:  R Jacobs; M G Butler; D L Scott
Journal:  Clin Rheumatol       Date:  1990-12       Impact factor: 2.980

2.  The topoisomerase II inhibitor VM-26 induces marked changes in histone H1 kinase activity, histones H1 and H3 phosphorylation, and chromosome condensation in G2 phase and mitotic BHK cells.

Authors:  M Roberge; J Th'ng; J Hamaguchi; E M Bradbury
Journal:  J Cell Biol       Date:  1990-11       Impact factor: 10.539

  2 in total

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