Synthesis of alpha-amylase and alpha-glucosidase by membrane bound ribosomes from Bacillus licheniformis.

Alpha-glucosidase was membrane bound during exponential growth of Bacillus licheniformis but was released into the medium during stationary phase. It could be partially removed from exponential phase cells by washing with NaCl (0.5 M). Alpha-Amylase was exclusively extracellular and could not be detected in cells. Polysomes were prepared from exponential phase cells and separated into membrane bound and soluble fractions. In vitro chain completion and immunoprecipitation showed that alpha-glucosidase and alpha-amylase were synthesized by membrane bound and not by soluble ribosomes.