Literature DB >> 6192087

Identification of an antigen-specific immunoglobulin M antibody associated with acute Toxoplasma infection.

H A Erlich, G Rodgers, P Vaillancourt, F G Araujo, J S Remington.   

Abstract

We used the protein gel transfer technique (Western blotting) to analyze the immune response to individual electrophoretically resolved Toxoplasma antigens. Toxoplasma antigens were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The array of resolved proteins was electrophoretically transferred to CNBr-activated filter paper and incubated with serum samples from patients with either acute or chronic Toxoplasma infections or with serum samples from seronegative controls. Serum immunoglobulin G and immunoglobulin M bound to individual Toxoplasma antigens were detected by radioiodinated staphylococcal protein A and a goat anti-human immunoglobulin M antibody, respectively. A complex array of Toxoplasma antigens was revealed by this procedure. Immunoglobulin M directed against a low-molecular-weight antigen was detected in sera from all acutely infected adults (19 sera tested) and was absent from all sera from individuals with chronic infections (9 sera tested) and from sera from seronegative controls (9 sera tested).

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6192087      PMCID： PMC264696          DOI： 10.1128/iai.41.2.683-690.1983

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  12 in total

1.  The diagnosis of toxoplasmosis.

Authors:  S E Anderson; J S Remington
Journal:  South Med J       Date:  1975-11       Impact factor: 0.954

2.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

3.  Value of lymph-node biopsy in the diagnosis of acute acquired toxoplasmosis.

Authors:  R F Dorfman; J S Remington
Journal:  N Engl J Med       Date:  1973-10-25       Impact factor: 91.245

4.  A sensitive radioimmunoassay for detecting products translated from cloned DNA fragments.

Authors:  H A Erlich; S N Cohen; H O McDevitt
Journal:  Cell       Date:  1978-04       Impact factor: 41.582

5.  Electrophoretic transfer of proteins and nucleic acids from slab gels to diazobenzyloxymethyl cellulose or nitrocellulose sheets.

Authors:  M Bittner; P Kupferer; C F Morris
Journal:  Anal Biochem       Date:  1980-03-01       Impact factor: 3.365

6.  Filter affinity transfer. A new technique for the in situ identification of proteins in gels.

Authors:  H A Erlich; J R Levinson; S N Cohen; H O McDevitt
Journal:  J Biol Chem       Date:  1979-12-10       Impact factor: 5.157

7.  Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure.

Authors:  J Renart; J Reiser; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205

8.  Serological and immunochemical characterization of monoclonal antibodies to Toxoplasma gondii.

Authors:  E Handman; J S Remington
Journal:  Immunology       Date:  1980-08       Impact factor: 7.397

9.  An enzyme-linked immunosorbent assay for detection of IgM antibodies to Toxoplasma gondii: use for diagnosis of acute acquired toxoplasmosis.

Authors:  Y Naot; J S Remington
Journal:  J Infect Dis       Date:  1980-11       Impact factor: 5.226

10.  Failure to trigger the oxidative metabolic burst by normal macrophages: possible mechanism for survival of intracellular pathogens.

Authors:  C B Wilson; V Tsai; J S Remington
Journal:  J Exp Med       Date:  1980-02-01       Impact factor: 14.307
View more
  15 in total

1.  Identification of Mycobacterium tuberculosis antigens in Seibert fractions by immunoblotting.

Authors:  S R Coates; D Hansen; G Schecter; G Slutkin; P Hopewell; L Affronti; D F Echenberg
Journal:  J Clin Microbiol       Date:  1986-07       Impact factor: 5.948

2.  A 38-kD Mycobacterium tuberculosis antigen associated with infection. Its isolation and serologic evaluation.

Authors:  C Espitia; I Cervera; R González; R Mancilla
Journal:  Clin Exp Immunol       Date:  1989-09       Impact factor: 4.330

3.  The infection-stage-related IgG response to Toxoplasma gondii studied by immunoblotting.

Authors:  C Verhofstede; P Van Gelder; M Rabaey
Journal:  Parasitol Res       Date:  1988       Impact factor: 2.289

4.  Kinetics study and characterisation of target excreted/secreted antigens of immunoglobulin G, M, A and E antibodies from mice infected with different strains of Toxoplasma gondii.

Authors:  P Cazabonne; M H Bessieres; J P Seguela
Journal:  Parasitol Res       Date:  1994       Impact factor: 2.289

5.  Method to map antigenic determinants recognized by monoclonal antibodies: localization of a determinant of virus neutralization on the feline leukemia virus envelope protein gp70.

Authors:  J H Nunberg; G Rodgers; J H Gilbert; R M Snead
Journal:  Proc Natl Acad Sci U S A       Date:  1984-06       Impact factor: 11.205

6.  Kinetics and time dependence of the differential agglutination of acetone [AC]- and formalin [HS]-fixed Toxoplasma tachyzoites by serum of mice with experimental toxoplasmosis.

Authors:  Nehad Mahmoud Ali; Khaled Sayed Mohamed Habib
Journal:  J Parasit Dis       Date:  2012-01-20

7.  Improved diagnosis of reactivated toxoplasmosis.

Authors:  D Ashburn; M M Davidson; A W Joss; T H Pennington; D O Ho-Yen
Journal:  Mol Pathol       Date:  1998-04

8.  Partially purified antigen preparations of Toxoplasma gondii protect against lethal infection in mice.

Authors:  F G Araujo; J S Remington
Journal:  Infect Immun       Date:  1984-07       Impact factor: 3.441

9.  Comparison of IgG antibody profiles by immunoblotting in patients with acute and previous Toxoplasma gondii infection.

Authors:  I L Moir; M M Davidson; D O Ho-Yen
Journal:  J Clin Pathol       Date:  1991-07       Impact factor: 3.411

10.  Antigen(s) responsible for immunoglobulin G responses specific for the acute stage of Toxoplasma infection in humans.

Authors:  Y Suzuki; P Thulliez; G Desmonts; J S Remington
Journal:  J Clin Microbiol       Date:  1988-05       Impact factor: 5.948
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.