Literature DB >> 6190650

Aberrant migration of lipopolysaccharide in sodium dodecyl sulfate/polyacrylamide gel electrophoresis.

P J Hitchcock.   

Abstract

Purified lipopolysaccharides of salmonellae strains were separated by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Pre-electrophoresis of polyacrylamide gels had no apparent effect on one-dimensional silver-stained lipopolysaccharide profiles. However, without pre-electrophoresis, two-dimensional and three-dimensional patterns contained numerous bands with varied migration patterns compared to those in the one-dimension gels. The lipopolysaccharide was altered within the polyacrylamide gel during electrophoresis. Pre-electrophoresis of gels eliminated aberrant migration patterns.

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Year:  1983        PMID： 6190650     DOI： 10.1111/j.1432-1033.1983.tb07517.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  23 in total

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