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Literature DB >> 6190130

Mouse hepatic metallothionein-I gene cleavage by micrococcal nuclease is enhanced after induction by cadmium.

J Koropatnick, G Andrews, J D Duerksen, U Varshney, L Gedamu.

Abstract

Micrococcal nuclease has been shown to preferentially cleave chromatin in the region of genes actively engaged in transcription. We have used this preferential cleavage to show that the metallothionein (MT) gene in adult mouse liver, when induced to produce mRNA by injection of cadmium, becomes more susceptible to nuclease cleavage. However, the MT gene in uninduced liver, and the alphafoetal protein (AFP) gene in both induced and uninduced liver, remain relatively resistant to nuclease cleavage. The AFP gene is not normally expressed in cadmium induced or uninduced liver. Thus, susceptibility of genes to nuclease cleavage appears to rise with increasing transcription of the gene.

Entities: Chemical Gene Species

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Year: 1983 PMID： 6190130 PMCID： PMC325961 DOI： 10.1093/nar/11.10.3255

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

30 in total

5. DNase I hypersensitivity and methylation of the 5'-flanking region of the alpha 1-fetoprotein gene during developmental and glucocorticoid-induced repression of its activity in rat liver.

Authors: B Turcotte; M Guertin; M Chevrette; H LaRue; L Bélanger
Journal: Nucleic Acids Res Date: 1986-12-22 Impact factor: 16.971

5 in total

Mouse hepatic metallothionein-I gene cleavage by micrococcal nuclease is enhanced after induction by cadmium.

1. Synthesis of metallothionein in a polysomal cell-free system.

2. Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.

3. Ribosomal genes of Xenopus laevis: evidence of nucleosomes in transcriptionally active chromatin.

4. Chromosomal subunits in active genes have an altered conformation.

5. Analysis and reconstruction of Xenopus ribosomal chromatin nucleosomes.

6. Genes transcribed at diverse rates have a similar conformation in chromatin.

Review 7. Alpha-fetoprotein in ontogenesis and its association with malignant tumors.

8. Kinetics of cadmium-induced hepatic and renal metallothionein synthesis in the mouse.

9. A membrane-filter technique for the detection of complementary DNA.

10. Sites of serum alpha-fetoprotein synthesis in the human and in the rat.

1. Differential repair of DNA damage in the human metallothionein gene family.

2. Acute treatment of mice with cadmium salts results in amplification of the metallothionein-1 gene in liver.

3. A mutant mouse (tx) with increased hepatic metallothionein stability and accumulation.

4. Butyrate-induced changes in nuclease sensitivity of chromatin cannot be correlated with transcriptional activation.

5. DNase I hypersensitivity and methylation of the 5'-flanking region of the alpha 1-fetoprotein gene during developmental and glucocorticoid-induced repression of its activity in rat liver.