| Literature DB >> 6190128 |
R C Bleackley, H Horak, J McElhaney, C Havele, A R Shaw, A R Turner, V H Paetkau.
Abstract
Poly A+ RNA has been purified from phorbol myristate acetate stimulated mouse EL4 cells. Translation, by microinjection into frog oocytes, gave biologically active interleukin 2 (IL2) and colony stimulating factor for granulocytes and macrophages (GM-CSF). These two mRNAs were separated by centrifugation through linear sucrose gradients. The sedimentation coefficients for IL2 and GM-CSF mRNAs were found to be 11.5S and 8S respectively. The clonal and cellular morphologies induced by the oocyte material corresponded to low concentrations of authentic GM-CSF.Entities:
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Year: 1983 PMID： 6190128 PMCID： PMC325945 DOI： 10.1093/nar/11.10.3027
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971