Literature DB >> 6189486

Nucleotide sequence of the promoter and NH2-terminal signal peptide region of Bacillus subtilis alpha-amylase gene cloned in pUB110.

K Ohmura, H Yamazaki, Y Takeichi, A Nakayama, K Otozai, K Yamane, M Yamasaki, G Tamura.   

Abstract

The nucleotide sequence of the promotor and NH2-terminal signal peptide region of the alpha-amylase gene derived from the alpha-amylase hyperproducing strain B. subtilis NA64 was determined. DNA sequences of the NH2-terminal region of the mature alpha-amylase, 41 amino acid residues of the signal peptide, a Shine-Dalgarno sequence (AGGAG), a potential RNA polymerase recognition site (TTGAAA), and a potential Pribnow box (AAGTAA) were identified. The DNA sequence was quite different from that of the alpha-amylase gene of B. amyloliquefaciens.

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Year:  1983        PMID： 6189486     DOI： 10.1016/0006-291x(83)91516-4

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  6 in total

1.  Nucleotide sequence of the type A streptococcal exotoxin (erythrogenic toxin) gene from Streptococcus pyogenes bacteriophage T12.

Authors:  C R Weeks; J J Ferretti
Journal:  Infect Immun       Date:  1986-04       Impact factor: 3.441

Review 2.  Protein secretion in Bacillus species.

Authors:  M Simonen; I Palva
Journal:  Microbiol Rev       Date:  1993-03

Review 3.  Compilation of published signal sequences.

Authors:  M E Watson
Journal:  Nucleic Acids Res       Date:  1984-07-11       Impact factor: 16.971

4.  In vivo selected promoter and ribosome binding site up-mutations: demonstration that the Escherichia coli bla promoter and a Shine-Dalgarno region with low complementarity to the 16 S ribosomal RNA function in Bacillus subtilis.

Authors:  A Hung; J Thillet; R Pictet
Journal:  Mol Gen Genet       Date:  1989-10

5.  Length and structural effect of signal peptides derived from Bacillus subtilis alpha-amylase on secretion of Escherichia coli beta-lactamase in B. subtilis cells.

Authors:  K Ohmura; K Nakamura; H Yamazaki; T Shiroza; K Yamane; Y Jigami; H Tanaka; K Yoda; M Yamasaki; G Tamura
Journal:  Nucleic Acids Res       Date:  1984-07-11       Impact factor: 16.971

6.  Alpha-amylase genes (amyR2 and amyE+) from an alpha-amylase-hyperproducing Bacillus subtilis strain: molecular cloning and nucleotide sequences.

Authors:  H Yamazaki; K Ohmura; A Nakayama; Y Takeichi; K Otozai; M Yamasaki; G Tamura; K Yamane
Journal:  J Bacteriol       Date:  1983-10       Impact factor: 3.490
  6 in total

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