Transganglionic demonstration of central sensory projections from skin and muscle with HRP-lectin conjugates.

Transganglionic transport of horseradish peroxidase (HRP) was greatly improved by conjugating the enzyme with wheat germ agglutinin (WGA) as described by Gonatas et al. [7]. Intense dorsal horn projections from muscle groups and even discrete skin patches were labeled after peripheral injection of the HRP-WGA conjugate. These projections could not be consistently demonstrated when HRP was injected either alone or in combination with DMSO. Conjugation with WGA thus enhances the uptake and/or transport of HRP and constitutes a valuable advance in HRP technique.